鲢鱼降血压肽的酶解条件  

Study on Manufacture of Enzymolyzed Antihypertensive Peptide Abstracted from Silver Carp

作　　者：毋瑾超[1] 汪依凡[1] 方长富[1] 

机构地区：[1]国家海洋局第二海洋研究所,浙江杭州310012

出　　处：《湛江海洋大学学报》2006年第1期36-39,共4页Journal of Zhanjiang Ocean University

基　　金：国际科学基金IFS和OPCW资助项目(E/3352-1)

摘　　要：以鲢鱼蛋白为原料,采用5种不同复合蛋白酶(P1,P2,P3,P4,P5)进行酶解,综合研究了复合生物酶酶量、酶解时间、pH、酶解温度等对鲢鱼蛋白酶解降血压肽ACE活性抑制效果的影响。结果表明,复合酶P1酶解降血压肽对ACE抑制率经体外检测为88.08%,具有最佳的酶解效果。对应最佳条件为:酶量25 IU/mL,酶解时间2 h,pH7.0,酶解温度60℃。This paper deals with the effects of the quantity of enzyme concentration, hydrolysis temperature, pH and hydrolysis duration of 5 different complex enzymes on the ACE（Angiotensin-converting enzyme） inhibitory activity of antihypertensive peptide abstracted from silver carp. The results are as follows： enzyme PI has the best hydrolysis result, and the ACE inhibitory ratio of the antihypertensive peptide is 88.08 % in vitro. The suitable hydrolysis conditions of this certain complex enzyme are given with enzyme concentration of 25 IU/mL, hydrolysis duration of 2 h, pH of 7.0, hydrolysis temperature of 60 ℃.

关 键 词：降血压肽 酶解 ACE抑制活性 鲢鱼 

分 类 号：Q819[生物学—生物工程]