利用高效液相色谱法测定雨生红球藻中虾青素含量  

Determination of Astaxanthin in Haematocoocus Pluvialls by HPLC

作　　者：赵立艳[1] 赵广华[1] 陈芳[1] 吴继红[1] 胡小松[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《食品工业科技》2006年第5期177-178,共2页Science and Technology of Food Industry

基　　金：国家863项目。

摘　　要：利用高效液相色谱法对雨生红球藻中的虾青素含量进行了测定。色谱柱为KromasilC18柱(250×4.6mm),流动相为二氯甲烷∶甲醇∶乙腈∶水=5∶85∶5∶5,流速1mL/min,检测波长480nm,柱温25℃。虾青素在1.896～18.96μg/mL范围内线性关系良好,r=0.9999,RSD=1.12%(n=6),回收率为99.69%～102.6%。本方法简便快速,定量准确,重现性好。An HPLC method was developed for the determination of astaxanthin from Haematococcus Pluvialis. The chromatography condition was： Kromasil C18（250μ4.6mm） column, a mixture of dichloromethane, methanol, acetonitrile and water （5：85：5：5）as mobile phase, flow rate of 1mL/min, detect wavelength at 480nm, column temperature at 25℃ . A good linearity was shown in the concentration range of 1.896-18.96 μg/mL （r= 0.9999）,RSD was 1.12%（n=6）,and the recovery was 99.74%-102.05%. The method has been proven to be convenient, quick, accurate and with good reproducibility.

关 键 词：高效液相色谱 雨生红球藻 虾青素 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]