一种新型产纤维素酶菌种的分离鉴定  

Isolation and identification of a novel cellulase-producing fungus

作　　者：余永红[1] 邓泽元[1] 范亚苇[1] 刘文群[1] 邓胜国[1] 

机构地区：[1]南昌大学食品科学教育部重点实验室,南昌大学食品科学与工程系,江西南昌330047

出　　处：《食品工业科技》2006年第6期52-53,共2页Science and Technology of Food Industry

基　　金：江西省教育厅2005研究项目(2005026)

摘　　要：从江西生产豆渣菌的作坊采样,检测到一株分解纤维素的真菌(MC1),分离纯化后,再利用以纤维素为唯一碳源的选择性培养基培养,根据形态特征可鉴定为面包串珠霉(MoniliasitophilaMont.)。摇床发酵产酶实验表明,在30℃、160r/min的条件下摇瓶培养6d后,其所产纤维素酶系中的以羧甲基纤维素钠为底物的CMC酶活为4119U/mL,以微晶纤维素为底物的C1酶活为6833U/mL,以水杨素为底物的βG酶活为451U/mL。A fungus,producing cellulase and growing in the residue from Dofu processing was isolated. The fungus was identified as Monilia Sitophila Mont. according to its morphological characteristics. The results showed that the fungus produced 3 cellulases whose CMC enzyme activity was 4119U/mL on carboxymethyl cellulose-Na, C1 enzyme activity was 6833 U/mL on crystallite cellulose, βG enzyme activity was 451 U/mL on salicin after submerged culture （160r/min） for 6 days at 30℃.

关 键 词：面包串珠霉 分离 鉴定 纤维素酶 

分 类 号：TS201.25[轻工技术与工程—食品科学]