γ-聚谷氨酸产生菌S004-50-01的筛选和优化培养  

Screening of Bacillus natto S004-50-01 Producing γ-Polyglutamic Acid and Optimization of Fermentation Culture

作　　者：李楠[1] 黄登禹 李飞[3] 林旭辉[1] 张坤生[1] 刘丽娟[1] 王佳玮[1] 李娜[1] 郭琳琳[1] 金燕[1] 邹巧云[1] 

机构地区：[1]天津商学院食品科学与工程系,天津300134 [2]天津市百奥生物技术有限公司,天津300221 [3]天津科技大学生物技术学院,天津300222

出　　处：《食品与发酵工业》2006年第6期1-4,共4页Food and Fermentation Industries

基　　金：天津市科委鉴定项目(津科成鉴字[2005]第258号)

摘　　要：以纳豆芽孢杆菌(Bacillus natto)S004为出发菌,经诱变选育后得到S004-50-01菌株,聚谷氨酸产量由原菌株的4.25 g/L提高到10.0 g/L,对谷氨酸的利用率由18.2%提高到42.83%。通过正交实验,获得该菌株的优化培养条件为:葡萄糖40 g/L,谷氨酸钠30 g/L,MgSO40.5 g/L,FeCl30.5 g/L,MnSO40.02 g/L,K2HPO43.0 g/L,经72 h发酵培养,聚谷氨酸的平均产量为13.6 g/L,谷氨酸的利用率为45.33%。In the present study, Bacillus natto S004-50-01 was screened from its parent strain of Bacillus natto S004 by ultraviolet radiation. γ-Polyglutamic acid yield of the strain increased from original 4.25 g/L to 10.0 g/L, the utilization ratio of glutamic acid enhanced from original 18.2% to 42.83%. The optimal result was obtained that the culture medium was glucose 40 g/L, sodium glutamate 30 g/L, MgSO4 0.5 g/L, FeCL3 0.5 g/L, MnSO4 0.02 g/L, and K2HPO4 3.0 g/L by orthogonal experiment. After fermented cultivation in 72 hours, the average yield of γ-Polyglutamic acid was 13.6 g/L, the utilization ratio of glutamic acid was 45.33 %.

关 键 词：Γ-聚谷氨酸 纳豆芽孢杆菌 筛选 优化培养 

分 类 号：TQ922.1[轻工技术与工程—发酵工程]