菜籽肽抗氧化活性的研究  

Antioxidant activity of rapeseed peptide(RSP)

作　　者：薛照辉[1] 尉万聪[2] 严奉伟[3] 吴谋成[3] 周志江[1] 

机构地区：[1]天津大学农业与生物工程学院,天津市300072 [2]中国农业科学院生物技术研究所,北京市100081 [3]华中农业大学食品科技学院,武汉市430072

出　　处：《中国油脂》2006年第8期48-50,共3页China Oils and Fats

基　　金：天津大学青年教师基金(5110107);国家"十五"科技重点攻关计划(2001BA501A05)

摘　　要：用分光光度法测定大鼠红细胞溶血程度,用丙二醛(MDA)试剂盒测定MDA。结果显示,通过腹膜注射菜籽肽,可以使小鼠血清中MDA含量显著减少。高剂量组[100 mg/(kg.d)]与对照组相比,MDA的降低达到极显著水平(P<0.01)。离体实验结果显示,菜籽肽粗品及各级分对体外温育和H2O2诱导的小鼠肝组织匀浆MDA生成有较强的抑制作用,其中粗品RSP-R和级分RSP-1的作用最好,且有量效关系,而对Fe2+诱导的MDA生成的抑制作用稍差。另外,RSP-R还可抑制大鼠红细胞氧化溶血程度。以上结果表明,菜籽肽在体内外均具有明显的抗氧化作用。The effect of RSP on hemolysis of mice red blood cell was observed in the spectrophotometric method, while the reagent kits were used to analyse the content of malondialdehyde （ MDA ）. The results showed that MDA levels in serum in the RSP group were obviously lower than that of the control after injecting RSP for 12 d, there was a significant difference（ P 〈 0. 01 ） between the control group and the intra - peritoneal injected group （with RSP 100 mg/（ kg · d） 12 d） in MDA value in mice serum. In vitro, as for malondialdehyde（MDA） formation and H2O2 - induced of mice liver ,RSP - R and RSP - 1 had better inhibiting effects than RSP -2 and RSP - 3 , exhibiting dosage -depended, but little inhibiting effect on MDA induced by Fe^2 ＋. RSP -R can also inhibiting the hemolysis of mice red blood cell as well. All the results above show that RSP has antioxidation effect in vitro and in vivo.

关 键 词：菜籽清蛋白 菜籽肽 抗氧化 体内 体外 

分 类 号：TQ645[化学工程—精细化工]