Caffeic acid ameliorates early and delayed brain injuries after focal cerebral ischemia in rats  

作　　者：Yu ZHOU San-hua FANG Yi-lu YE Li-sheng CHU Wei-ping ZHANG Meng-ling WANG Er-qing WEI 

机构地区：[1]Department of Pharmacology School of Medicine, Zhejiang University, Hangzhou 310031, China

出　　处：《Acta Pharmacologica Sinica》2006年第9期1103-1110,共8页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of China(№30371637);the Scientific Foundation of Education Ministry of China(№20050335105).

摘　　要：Aim： To investigate the effects of caffeic acid on early and delayed injuries after focal cerebral ischemia in rats, and the possible relation to 5-1ipoxygenase inhibition. Methods： Transient focal cerebral ischemia was induced by middle cerebral artery occlusion in Sprague-Dawley rats. Caffeic acid （10 and 50 mg/kg） was ip injected for 5 d after ischemia. The brain injuries were observed, and the levels of cysteinyl leukotrienes and leukotriene B4 in the brain tissue were measured. Results： Caffeic acid （50 mg/kg） ameliorated neurological dysfunction and neuron loss, and decreased infarct volume 24 h after ischemia; it attenuated brain atrophy, infarct volume, and particularly astrocyte proliferation 14 d after ischemia. In addition, it reduced the production of leukotrienes （5-1ipoxygenase metabolites） in the ischemic hemispheres 3 h and 7 d after ischemia. Conclusion： Caffeic acid has protective effect on both early and delayed injuries after focal cerebral ischemia in rats; and this effect may partly relate to 5-1ipoxygenase inhibition.Aim： To investigate the effects of caffeic acid on early and delayed injuries after focal cerebral ischemia in rats, and the possible relation to 5-1ipoxygenase inhibition. Methods： Transient focal cerebral ischemia was induced by middle cerebral artery occlusion in Sprague-Dawley rats. Caffeic acid （10 and 50 mg/kg） was ip injected for 5 d after ischemia. The brain injuries were observed, and the levels of cysteinyl leukotrienes and leukotriene B4 in the brain tissue were measured. Results： Caffeic acid （50 mg/kg） ameliorated neurological dysfunction and neuron loss, and decreased infarct volume 24 h after ischemia; it attenuated brain atrophy, infarct volume, and particularly astrocyte proliferation 14 d after ischemia. In addition, it reduced the production of leukotrienes （5-1ipoxygenase metabolites） in the ischemic hemispheres 3 h and 7 d after ischemia. Conclusion： Caffeic acid has protective effect on both early and delayed injuries after focal cerebral ischemia in rats; and this effect may partly relate to 5-1ipoxygenase inhibition.

关 键 词：caffeic acid focal cerebral ischemia NEURON ASTROCYTE 5-LIPOXYGENASE LEUKOTRIENE 

分 类 号：R742[医药卫生—神经病学与精神病学]