高效液相色谱法同时测定大豆粕四种异黄酮含量研究  

Study on Simultaneous Determination of Four Isoflavones from Soybean Meal By HPLC

作　　者：高荣海[1] 孙昭宁[2] 李长彪[1] 刘长江[1] 

机构地区：[1]沈阳农业大学食品学院,沈阳110161 [2]中国水产科学研究院,北京100039

出　　处：《粮食与油脂》2006年第10期33-35,共3页Cereals & Oils

摘　　要：该文介绍高效液相色谱同时测定大豆粕中四种大豆异黄酮方法。确定出色谱条件为:色谱柱Hypersil ODS2C18柱(250min×4．6mm ID,5μm),流动相:甲醇:水=40:60,柱温30℃,流速1．0ml／min,检测波长260 nm。大豆苷、染料木苷、大豆苷元、染料木素在0．21-8．56μg／mL范围内浓度与峰面积具有很好线性关系,相关系数r=0．999155-0．999991;该测定法回收率大于99％,变异系数小于3％。A method for simultaneous determination of four soybean isoflavone from soy meal by high performance liquid chromatography was described. The operating conditions were： The column was Hypersil ODS2 C18 （250 mm×4.6 mm ID, 5μm）, the mobile phase was MeOH ： H20 = 40 ： 60, the temperature of column was 30℃, the flow rate was 1.0 ml/min, the detection wavelength was 260 mn. The liner range of daidzin,genistin, daidzein and genistein was 0.21-8.56 μg/mL with a correlatione coeff-icient of 0.999155 - 0.999991. The average recovery rate was more than 99% with RSD less than 3%.

关 键 词：大豆异黄酮 高效液相色谱法 大豆粕 

分 类 号：TS202.3[轻工技术与工程—食品科学]