缓释葡萄糖氧化酶最适制备条件初探  

Pilot Study on the Optimum Conditions to Prepare Controlled-release Glucose Oxidase

作　　者：王学东[1] 辛锋[1] 夏文水[1] 沈炯[1] 郇美丽[1] 张莉萍[1] 

机构地区：[1]武汉工业学院食品科技学院,湖北武汉430023

出　　处：《食品科学》2006年第11期248-253,共6页Food Science

基　　金：湖北省自然科学基金项目(2005ABA090);湖北省教育厅重点项目(D200518007)

摘　　要：以壳聚糖和海藻酸钠为复合缓释壁材,对葡萄糖氧化酶(GOD)进行微胶囊包被缓释处理,研究了不同因素对缓释葡萄糖氧化酶酶活回收率和缓释作用的影响。结果显示,制备缓释葡萄糖氧化酶的最佳条件为:海藻酸钠浓度2.0%,壳聚糖浓度0.75%,葡萄糖氧化酶浓度0.3%,pH值5.0,钙离子浓度0.3mol/L,搅拌速度1200r/min。此时葡萄糖氧化酶酶活回收率最高,达到93.8%,且显示出较好的缓释性能。Chitosan/sodium alginat was selected as a compound wall material of controlled- release microcapsule of glucose oxidase in this study. Influence of different factors to the recovery of enzyme activity and the controUed-release process were investigated. The results showed that the optimal conditions to prepare the controlled- release glucose oxidase were concentration of sodium alginate 2.0%, concentration of chitosan 0.75%, concentration of glucose oxidase 0.6%, pH 5.0, concentration of calcium ion 0.3%, stirring speed 1200r/min. Under this condition the recovery of enzyme activity reached 93.8%, and the controUed-release GOD showed better slow release character.

关 键 词：葡萄糖氧化酶 壳聚糖/海藻酸钠 缓释 制备条件 

分 类 号：Q946.5[生物学—植物学]