繁茂膜海绵中产甲壳素酶菌株筛选的研究  

STUDIES ON STRAINS SCREEN OF PRODUCING CHITINASE FROM HYMENIACIDON PERLEVE

作　　者：朱妍妍[1] 黄剑宇[1] 牟光庆[2] 靳艳[1] 

机构地区：[1]中科院大连化学物理研究所,辽宁大连116023 [2]大连轻工业学院食物与食品工程学院

出　　处：《食品研究与开发》2006年第11期29-31,共3页Food Research and Development

基　　金：国家"973项目"(2003CB716001)

摘　　要：通过富集培养和初筛,从繁茂膜海绵中分离到108株产甲壳素酶菌株;其中有12株菌产生的透明圈比较明显。结合平板法和酶活力比较法,筛选出1株高酶活力的优良菌株H7,酶活力为0.54U/mL。通过16SrRNA同源序列分析和系统发育树分析,鉴定菌株H7为Pseudoalteromonas属,和P.ganghwensis\DQ011614相似性达到98%。108 strains of producing chitinase were isolated from Hymettiacidon perleve by enrichment culture and preliminary screening, and there were 12 strains can produce transparent circle obviously. One good strain H7 of producing high enzyme activity was screened by plate process amt enzyme activity comparison method.The enzyme activity of H7 is 0.54 U/mL.H7 was identified as Pseudoalteromaons by analyzing of homologization sequence of 16S rRNA and phylogenetic tree ,The similarity of H7 is 98 % with P, ganghwensis/DQ011614.

关 键 词：繁茂膜海绵 甲壳素酶 酶活力 筛选 交替假单胞菌 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]