UV、LiCl复合诱变深黄被孢霉选育γ-亚麻酸突变株  

Screening of A Producing γ-linolenic Acid Mutant Strain(Mortierella isabellina) by UV and LiCl

作　　者：魏娜[1] 李柏林[1] 欧杰[1] 蒋志斌[1] 

机构地区：[1]上海水产大学食品学院,上海200090

出　　处：《食品科学》2006年第12期201-203,共3页Food Science

基　　金：上海市重点学科建设项目(T1102)

摘　　要：以深黄被孢霉(Mortierellaisabellina)AS3.3410为出发菌株,经UV、LiCl复合诱变处理,随机筛选后复筛,最终获得一株突变株SHFU-13。其种子发酵2d,摇瓶发酵8d时,生物量和总脂量分别达21.66、12.65g/L,在同等条件下比出发菌株分别提高了8.84%和36.61%。气相色谱分析其油脂组成,γ-亚麻酸含量为6.39%,产量为0.81g/L,产量较对照样提高44.64%。连续传代多次,其产量性状无显著变化。A mutant strain SHFU-13 was successfully obtained when the original strain Morrierella isabeUina AS3.3410 was treated by UV and LiCl as mutating agent. The biomass was up to 21.66g/L, which was 8.84% higher than that of the original strain while the product of the total lipid was up to 12.65g/L, which was 36.61% higher than that in the original strain mycelium. Analysis by gas chromatograph indicated the content of GLA in the lipid was 6.39% while the product of it was up to 0.81g/L, which was 44.64% higher than that of the original strain. After passing on many times, there were no distinct changes on the production.

关 键 词：深黄被孢霉 Γ-亚麻酸 诱变 

分 类 号：TQ921.7[轻工技术与工程—发酵工程]