反相高效液相色谱-柱后衍生法分析检测鱿鱼中的生物胺  

Determination of Biogenic Amines in Squid by Reversed Phase High-Performance Liquid Chromatography and Post-Column Derivatization

作　　者：赵庆喜[1] 薛长湖[1] 徐杰[1] 盛文静[1] 薛勇[1] 李兆杰[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《食品与生物技术学报》2007年第3期14-19,共6页Journal of Food Science and Biotechnology

基　　金：国家863计划项目(2005AA625010)

摘　　要：建立了水产品中多组分生物胺的反相高效液相色谱-柱后衍生-荧光检测方法。采用荧光试剂邻苯二甲醛(OPA)为柱后衍生化试剂,在Capcell Pak MG-C18色谱柱上,通过梯度洗脱使酪胺、腐胺、尸胺、组胺、胍丁胺、精胺和亚精胺等7种生物胺得到良好分离,在给定的浓度范围内,各组分生物胺呈现良好线性相关(R2>0.999)。在水产品中添加生物胺混合标准溶液,平均回收率为88.3%～110.1%,相对标准偏差RSD小于10%。结合水产品的感官鉴定、pH值和TVBN值测定等方法检测水产食品的新鲜程度,分析了鱿鱼在不同保藏温度、保藏时间下的生物胺种类及含量的变化。其中胍丁胺和尸胺在鱿鱼的保藏过程中发生最显著变化,可以作为其质量变化的参考指标。A post-column derivatization reversed-phase high-performance liquid chromatography method was developed to the determination of biogenic amines in aquatic products with fluorescecse detector （FLD）. Seven biogenic amines （ tyramine, putrescine, cadaverine, histamine, agmatine, spermidine and spermine） were seperated well. The method was linear for the amines studied at given concentrations（R^2〉0. 999）, The average recoveries ranged from 88.3% to 110. 1% for all amines samples. The RSDs were less than 10%. Sensory analysis, pH value and TVBN value were combined to detect the freshness of aquatic products. With this method, agmatine and cadaverine in squid were the most obviously changing biogenic amines during the storage at different temperatures, and these biogenic amines could be the potential index for the freshness of these raw aquatic materials. In conclusion, the method could be applied for determination of biogenic amines in aquatic products.

关 键 词：高效液相色谱 柱后衍生 生物胺 鱿鱼 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]