宇佐美曲霉木聚糖酶基因在大肠杆菌中的表达与优化  

Expression of xylanase gene xynⅡ from Aspergillus usamii E001in Escherichia coil

作　　者：白剑宇[1] 周晨妍[1] 王瑾[1] 邬敏辰[1] 

机构地区：[1]江南大学生物工程学院,江苏无锡214036

出　　处：《食品工业科技》2007年第6期100-103,共4页Science and Technology of Food Industry

摘　　要：从宇佐美曲霉(Aspergillus usamii)E001中克隆木聚糖酶基因xynⅡ,将不含原酶信号肽编码序列的xynⅡ以正确的读码框克隆到大肠杆菌表达载体pET-28a中,构建成重组质粒pET-Xn并转化大肠杆菌,得到重组工程菌KN21。通过对其进行IPTG浓度、温度等诱导培养条件的优化后,胞内重组木聚糖酶活力最高达到81.77U/mg。对重组木聚糖酶进行酶学性质研究,显示其最适温度为45℃,最适pH为4.5。The gene xyn Ⅱ encoding the xylanase was cloned from Aspergillus usamii E001. The xyn Ⅱ without sequence encoding original signal peptide were cloned into the plasmid pET-28a in correct reading frame, then the recombinant plasmids pET-Xn was transformed into the host E. coli, and the recombinant strain KN21 was obtained. After optimization of induction, the expression level of xylanase exceeded 81.77 U/mg finally. The optimum temperature and pH of the recombinant xylanase were 45℃ and 4.5 respectively.

关 键 词：木聚糖酶 宇佐美曲霉 大肠杆菌 重组蛋白 高效表达 

分 类 号：TS201.25[轻工技术与工程—食品科学]