非衍生化HPLC法分析银耳多糖中单糖组成的初步研究  

Analysis of Monosacchaaride Composition in Tremella Polysaccharides by Non-derivatization High Performance Liquid Chromatography

作　　者：颜军[1] 郭晓强[2] 邬晓勇[1] 徐光域[2] 胡晓晓[1] 谭周军[1] 苟小军[1] 

机构地区：[1]成都大学药用微生物重点实验室,四川成都610106 [2]成都大学中药化学实验室,四川成都610106

出　　处：《食品科学》2007年第7期446-449,共4页Food Science

基　　金：四川省中医药管理局重点及专项项目(2003A001;2003C001);成都大学校基金项目

摘　　要：本研究采用非衍生化高效液相色谱法,以分离度与峰形两个因素作为评价指标,建立了鼠李糖、葡萄糖、果糖、木糖、半乳糖五种常见单糖的最佳分离条件。并应用所建立的方法对银耳多糖中的单糖组成进行了分析。初步确定银耳多糖中含有鼠李糖、木糖、果糖和葡萄糖,摩尔比为0.29:1.00:1.01:1.78。为此法在多糖的单糖组成测定中的更好运用提供了依据。This article is discussing a simple and sensitive high performance liquid chromatographic method（HPLC） for the analysis of monosacchoride composition in polysaccharides. Five monosaccharides arc separated by non-derivatization HPLC with RI monitor. By means of HPLC analysis rhanmose, glucose, fructose, xylose, galactose five kinds of monosaccharides, the best separation condition of the monosaccharide is obtained. The monosaccharide composition of Tremella polysaccharide is determined by a data analysis method which is developed to ensure accuracy and reproducibility.

关 键 词：HPLC 银耳多糖 单糖 

分 类 号：TS207.3[轻工技术与工程—食品科学]