变质豆浆中腐败微生物的分离与初步鉴定  

Separation and Preliminary Identification of Spoilage Organisms in Transmutative Soy Milk

作　　者：汪立平[1] 张庆华[1] 赵勇[1] 陈有容[1] 齐凤兰[1] 张闻[1] 

机构地区：[1]上海水产大学,上海200090

出　　处：《微生物学通报》2007年第4期621-624,共4页Microbiology China

基　　金：上海市教育基金会及上海科技成果转化促进会资助项目;上海市重点学科建设资助项目(No.T1102);上海市教委青年基金项目(No.04KC13)。

摘　　要：从5种变质豆浆中分离得到3株腐败菌株S1、S2和S3。3株菌均能在1×10^5Pa、30min杀菌条件下和添加300mg/kgNisin杀菌条件下存活。对分离菌株进行了菌落形态、生理生化特征和16SrDNA序列分析,鉴定结果表明3株菌均为革兰氏阴性细菌,分别为地衣芽孢杆菌（Bacillus licheniformis）、短小芽孢杆菌（Bacillus pumilus）和短芽孢杆菌（Brevibacillus borstelensis）,GenBank登录号为EF439666-EF439668。In this paper, three spoilage organisms were separated from five transmutative soy milks, and all the three spoilage boetena could survive condition of both 1 × 10^5Pa, 30min and 300mg/kg Nisin. Morpha character, physiological and biochemical characteristics, and a phylogenetic analysis based on 16S rDNA gene sequences reveal that these three strains are Bacillus licheniformis, Bacillus pumilus and BrevibaciUus borstelensis respectively. GenBank accessions for these three strains are EF439666-EF439668.

关 键 词：豆浆 腐败微生物 分离 鉴定 生理生化特征 16S RDNA 

分 类 号：TS201.3[轻工技术与工程—食品科学]