纤维素酶处理菠萝果渣提高粗多酚溶出率的研究  

Study on the Extraction of Polyphones from Pineapple Pomace by Cellulase

作　　者：范凤玲[1] 薛毅[1] 张金泽[1] 

机构地区：[1]中国食品发酵工业研究院,北京100027

出　　处：《食品与发酵工业》2007年第8期72-77,共6页Food and Fermentation Industries

基　　金：科技部科研院所专项基金(No.NCSTE-2006-JKZK-006)

摘　　要：以干燥的菠萝果渣为实验原料,在浸提温度、浸提pH、加酶量、漫提时间4个单因素试验的基础上,利用Box-Benhnken中心组合实验和响应面分析法优化纤维素酶处理菠萝果渣提取多酚类物质的工艺条件。通过三因素三水平的响应面分析,确定了纤维素酶处理菠萝果渣提取多酚类物质的理想工艺条件为:浸提时间140 min,漫提温度53℃,漫提pH3.8,酶剂量13 U/mL,此条件下粗多酚的溶出率为11.56 g/kg,比同条件下直接用水漫提提高了28.15%。Using dried pineapple pomace as experimental material and based on single tests including the factors of temperature, pH, enzyme dosage and time, the optimum conditions for the extraction of polyphenols from pineapple pomace treated with cellulase were obtained by Box-Benhnken center-united experiment design and Response Surface Analysis（RSA）. Through the analysis of tests with three factors and three levels,the optimum conditions were determined： extraction time 140min, extraction temperature 53℃, enzyme dosage 13U/mL. Under such condition, the extraction yield of polyphenols was 11.56g/kg,it was 28. 15% higher than the water extraction under the same condition.

关 键 词：菠萝果渣 纤维素酶 多酚 响应面分析 

分 类 号：TS201[轻工技术与工程—食品科学]