高效液相色谱法分析大豆和大豆低聚糖浆中糖的组成与含量  

Analysis of Free Sugars in Soybean Seed and Soybean Oligosaccharide Syrup by High Performance Liquid Chromatography

作　　者：向小丽[1] 杨立怡[1] 孙怡[1] 叶红[1] 麻浩[2] 曾晓雄[1] 

机构地区：[1]南京农业大学食品科技学院,江苏南京210095 [2]南京农业大学农学院,江苏南京210095

出　　处：《食品科学》2007年第12期412-415,共4页Food Science

摘　　要：采用高效液相色谱法(HPLC)对大豆种子及市售的大豆低聚糖浆中的可溶糖进行了分析,并利用α-半乳糖苷酶酶解大豆低聚糖浆及结合HPLC分析来推测大豆低聚糖浆的糖组成。HPLC分析条件为:Sugar-D色谱柱,75%乙腈为流动相,柱温40℃,流速为1.0ml/min,示差折光检测器。Sugar-D色谱柱的分离效果好,基线稳定,相对标准偏差1.0%～2.0%,标准回收率96.7%～100.7%。分析结果表明大豆种子和市售的大豆低聚糖浆中均含有果糖、蔗糖、蜜二糖、棉子糖、水苏糖以及二种保留时间介于蔗糖与蜜二糖之间的未知成分,而大豆低聚糖浆中还含有葡萄糖(半乳糖)和甘露三糖;大豆种子中主要含有蔗糖和水苏糖,而大豆低聚糖浆中含有较多的蔗糖、果糖、水苏糖和葡萄糖(半乳糖),功能性低聚糖主要为水苏糖和甘露三糖。The compositions of free sugars in soybean seeds and soybean oligosaccharide syrup were investigated through high performance liquid chromatography and enzymatic hydrolysis with β-galactosidase. A Sugar-D column was used with acetonitrile-water solution （75：25, V/V） as the mobile phase at a flow rate of I ml/min, and a refractive index detector was used for the detection of sugars. Under such conditions, it ensured good separation for the sugars with stable baseline. The relative standard deviations and recovery rates were between 1.0% to 2.0% and 96.7% to 106.7%, respectively, for fructose, sucrose, melibiose, raffinose, and stachyose. Through the analysis, we found that both soybean seed and soybean oligosaccharide syrup contained fructose, sucrose, melibiose, raffinose, stachyose, and two unknown substances, while the latter also contained glucose （galactose） and another unknown compound which was inferred as manninotriose based on the result of α-galactosidase degradation. The growth activator of bifldobacterial in soybean oligosaccharide syrup was mainly manninotriose and stachyose.

关 键 词：大豆 大豆低聚糖 高效液相色谱法 Α-半乳糖苷酶 

分 类 号：TS207.3[轻工技术与工程—食品科学]