大豆油对瘤胃培养液中共轭亚油酸及氢化中间产物累积规律的影响  

Effects of Soybean Oil on Concentrations of Conjugated Linoleic Acid and Accumulation of Intermediates of Ruminal Biohydrogenation in vitro

作　　者：侯俊财[1] 刘艳平[1] 桂仕林[1] 霍贵成[1] 贾志远[2] 姜宁[2] 张永忠[2] 

机构地区：[1]东北农业大学乳品科学教育部重点实验室 [2]黑龙江八一农垦大学动物科技学院,大庆163319

出　　处：《畜牧兽医学报》2008年第4期449-454,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：2002年教育部跨世纪人才基金;“十五”国家西部开发科技行动基金(2002BA901A46)

摘　　要：试验采用批次培养法研究了体外条件下添加大豆油对山羊瘤胃培养液中共轭亚油酸及氢化中间产物累积规律的影响,大豆油的添加量为0和100 mg/瓶。结果表明大豆油组cis-9,trans-11 CLA含量始终显著高于对照组(P<0.05),且在培养8 h时含量达到最高,为6.05 mg/g干物质;大豆油组trans-10,cis-12 CLA含量显著高于对照组(P<0.05),其含量随培养时间的延长而逐渐升高;在培养16 h时,大豆油组总共轭亚油酸含量达到最大,为11.61 mg/g干物质;大豆油组的trans-10C18∶1和trans-11C18∶1含量显著高于对照组(P<0.05),在培养过程中,二者含量始终增加。大豆油组总反式C18∶1脂肪酸含量随着培养时间的延长而逐渐增加,而总顺式C18∶1脂肪酸含量随着培养时间的延长而逐渐下降。This study was conducted to investigate the effect of addition of soybean oil in dietary on concentrations of conjugated linoleic acid and intermediates of ruminal biohydrogenation of mature goat in vitro by batch culture. The addition level of these soybean oil were 0（control group ） and 100 mg/flask, respectively. Results indicated that the cis-9, trans-11 CLA content of soybean oil supplementation treatment was significantly（P〈0.05）higher than that of the control all the time, and which reached its maximum after 8 hours＇ incubation （6.05 mg/g DM）. And the trans-10, cis-12 CLA content of soybean oil supplementation treatment was also significantly（P〈0.05）higher than that of the control all the time, and which increased gradually, with the extension of incubating time. The total CLA content of soybean oil supplementation treatment reached its maximum after 16 hours＇ incubation （11.61 mg/g DM）. During in vitro study, that trans-11 C18 ： 1 and trans-10 C18 ： 1 were accumulated with treatment of soybean oil, and the treatment was abstractly higher than the control （P〈0.05）. The total trans-C18 ： 1 content of soybean oil supplementation treatment increased gradually, with the extension of incubating time, while the total cis- C18 ： 1 content of soybean oil supplementation treatment decreased gradually.

关 键 词：大豆油 反-11C18:1 反-10C18:1 共轭亚油酸 瘤胃发酵 

分 类 号：S823.5[农业科学—畜牧学]