Effects of different concentrations of celecoxib on proliferation and cell cycle of SH-SY-5Y cells and its mechanisms In vitro study  

作　　者：Hu Zhou Wei Wang Hongyi Wang Jinghua Li 

机构地区：[1]Department of Hematology, Affiliated Hospital of Qingdao Medical College, Qingdao University, Qingdao 266003, Shandong Province, China [2]Department of Clinical Laboratory, Qingdao Municipal Hospital, Qingdao 266001, Shandong Province, China

出　　处：《Neural Regeneration Research》2008年第2期133-137,共5页中国神经再生研究（英文版）

基　　金：The Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry (2002)

摘　　要：BACKGROUND： Highly selective cyclooxygenase-2 （COX-2） inhibitors have recently been approved for the treatment of colon cancer, breast cancer, urinary bladder cancer, and skin cancer. For the highly selective COX-2 celecoxib, the mechanism of action for inhibiting neuroblastoma cells is still uncertain. OBJECTIVE： To observe the influence of different celecoxib concentrations on proliferation and cell cycle of SH-SY-5Y cells in vitro and to reveal potential COX-2-independent mechanisms of celecoxib on SH-SY-5Y cells. DESIGN： Controlled experiment. SETTING： Department of Hematology, Affiliated Hospital of the Qingdao Medical College, Qingdao University, Shandong Province. MATERIALS： The study was performed at the Cerebrovascular Disease Institute of Shandong Province and the Laboratory of Molecular Biology, Affiliated Hospital of Qingdao Medical College, Qingdao University between September 2006 and June 2007. The SH-SY-5Y cell line was obtained from the Department of Molecular Biology, Qingdao Medical College, Qingdao University. Celecoxib was obtained from Pfizer Pharmaceuticals LLC, USA. Coulter DNA PREP reagent kit was purchased from Beckman Coulter, Inc. The antibodies against human CyclinD1, P21, and P16 were purchased from Santa Cruz Biotechnology. METHODS： SH-SY-5Y cells were treated with different concentrations of celecoxib （10, 20, 40, and 80 la mol/L） for 48 hours and comprised the experimental groups. The same concentrations of DMSO （dimethyl sulphoxide） treatment for 48 hours served as the control group. MAIN OUTCOME MEASURES： ① Cellular morphology of cells pre-treated and post-treated with celecoxib by inverted microscopy. ② Methabenzthiazuron assay was used to measure cell proliferation. ③ Cell cycle was measured by flow cytometry after incubation with different celecoxib concentrations for 48 hours. ④CyclinD1, P16, and P21 protein expression was detected by Western blot analysis. RESULTS： ① Cellular morphology： The shape of SH-SY-5Y cells pre-treated with ceBACKGROUND： Highly selective cyclooxygenase-2 （COX-2） inhibitors have recently been approved for the treatment of colon cancer, breast cancer, urinary bladder cancer, and skin cancer. For the highly selective COX-2 celecoxib, the mechanism of action for inhibiting neuroblastoma cells is still uncertain. OBJECTIVE： To observe the influence of different celecoxib concentrations on proliferation and cell cycle of SH-SY-5Y cells in vitro and to reveal potential COX-2-independent mechanisms of celecoxib on SH-SY-5Y cells. DESIGN： Controlled experiment. SETTING： Department of Hematology, Affiliated Hospital of the Qingdao Medical College, Qingdao University, Shandong Province. MATERIALS： The study was performed at the Cerebrovascular Disease Institute of Shandong Province and the Laboratory of Molecular Biology, Affiliated Hospital of Qingdao Medical College, Qingdao University between September 2006 and June 2007. The SH-SY-5Y cell line was obtained from the Department of Molecular Biology, Qingdao Medical College, Qingdao University. Celecoxib was obtained from Pfizer Pharmaceuticals LLC, USA. Coulter DNA PREP reagent kit was purchased from Beckman Coulter, Inc. The antibodies against human CyclinD1, P21, and P16 were purchased from Santa Cruz Biotechnology. METHODS： SH-SY-5Y cells were treated with different concentrations of celecoxib （10, 20, 40, and 80 la mol/L） for 48 hours and comprised the experimental groups. The same concentrations of DMSO （dimethyl sulphoxide） treatment for 48 hours served as the control group. MAIN OUTCOME MEASURES： ① Cellular morphology of cells pre-treated and post-treated with celecoxib by inverted microscopy. ② Methabenzthiazuron assay was used to measure cell proliferation. ③ Cell cycle was measured by flow cytometry after incubation with different celecoxib concentrations for 48 hours. ④CyclinD1, P16, and P21 protein expression was detected by Western blot analysis. RESULTS： ① Cellular morphology： The shape of SH-SY-5Y cells pre-treated with ce

关 键 词：NEUROBLASTOMA CYCLOOXYGENASE CELECOXIB CYCLINS 

分 类 号：R749.1[医药卫生—神经病学与精神病学]