β-葡萄糖苷酶水解、分离纯化大豆异黄酮甙元工艺研究  

Study on isolating and purifying soy isoflavone aglycone by β-glucosidase hydrolysis

作　　者：汪海波[1] 黄爱妮[1] 李飞[1] 雷容[1] 汪海婴[2] 

机构地区：[1]武汉工业学院食品系,武汉430023 [2]华中科技大学同济医学院基础部,武汉430022

出　　处：《食品科技》2008年第4期114-119,共6页Food Science and Technology

基　　金：武汉市青年科技晨光计划项目(200750731286)

摘　　要：研究以低纯度的商品大豆异黄酮为原料,采用-β葡萄糖苷酶水解技术制备高纯度的大豆异黄酮甙元产品。比较系统地研究酶的用量、反应时间、反应温度、反应体系pH值、底物浓度等因素对酶水解效果的影响,最终得到酶水解制备工艺的最佳工艺参数。实验结果表明,采用该分离纯化工艺制备大豆异黄酮甙元产品,其产品得率、异黄酮回收率和产品纯度分别为12.12%、77.16%和92.78%,分离纯化效果比较理想。The high purification soy isoflavone aglycone was extracted from low purification soy isoflavone product by aether after β-glucosidase hydrolysis. In this study, the factors which influence to the enzyme hydrolysis, such as enzyme dosage, reaction time, reaction temperature, reaction system pH value, substrate concentration, solvent system and so on, are studied systematically and deeply. The experiment results indicate that the product obtains rate is 12.12%, the isoflavone returns-ratio is 77.16%, the product purity is 92.78% by using this β-glucosidase hydrolysis methods.

关 键 词：大豆异黄酮 Β-葡萄糖苷酶 水解 纯化 

分 类 号：TS201.1[轻工技术与工程—食品科学]