腐乳毛霉高产蛋白酶菌株的分离与鉴定  

Isolation and identification of high yield proteinase strain for sufu mucor

作　　者：程昌泽[1] 吴拥军[1] 龙菊[1] 王嘉福[1] 许文钊[1] 

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025

出　　处：《山地农业生物学报》2008年第2期127-133,共7页Journal of Mountain Agriculture and Biology

基　　金：贵阳市科技基金项目资助[(2005)筑科农字第20-7号]

摘　　要：从贵州各地风味较好的腐乳样品中分离到20株疑似毛霉菌株，经理化鉴定。并采用福林-酚试剂法检测产蛋白酶活力，获得1株发酵风味良好且蛋白酶活性较高的腐乳毛霉菌株MGC317。鉴定为雅致放射毛霉Actinomucor elegans（Eid）Benjam et Hesseh。将MGC317菌株接种麸皮培养基，28℃培养48h。其蛋白酶活性为57．26μL；对其16SrDNA的ITS序列进行克隆和分析，其分子长为711bp，与雅致放射毛霉比较。序列同源性为96％-97％。前发酵结果显示，MGC317菌株的菌丝高度为3．1cm。48h时蛋白酶活性为13.82μL。A total of 20 mucor doubtful strains were isolated from sufu material of Guizhou province. By identifying physically and chemically, one sufu mucor strain of well ferment flavour and productive proteinase activity was detected by Cefalexin-phenol reagent method,they are Actinomucor elegana（ Eid. ）Benjam. et Hesseh. MGC317 strain inoculated bran mash was cutivated at 28℃ in 48 hours, its proteinase activity was 57.26μL; 16SrDNA ITS sequence was cloned and analyzed,the length was 711bp. Comparing ITS sequence with Actinomucor elegarts strain, their sequence homology was 96%- 97% . To carry out sufu primary fermentation, its hypha height was 3. 1cm, its proteinase activity was 13. 82μ L at 48h. The results suggested that sufu mucor stralns with high accuracy could be used to identify ITS sequence of 16SrDNA. It was a valid way to obtain good strain.

关 键 词：腐乳 毛霉 蛋白酶 ITS 菌株分离 

分 类 号：TS201.3[轻工技术与工程—食品科学] TS214.3[轻工技术与工程—食品科学与工程]