欧姆加热对嗜酸耐热菌的电穿孔效应  

Electroporation Effect of Ohmic Heating on Alicyclobacillus acidoterrestris in Apple Juice

作　　者：耿敬章[1] 仇农学[2] 丁辉煌[2] 

机构地区：[1]陕西理工学院生物科学与工程学院,陕西汉中723001 [2]陕西师范大学食品工程系,陕西西安710062

出　　处：《食品与生物技术学报》2008年第3期79-82,共4页Journal of Food Science and Biotechnology

基　　金：国家“十五”重大专项资助课题(2001BA501A21);陕西理工学院引进人才项目(SLGQD0623)

摘　　要：探讨了欧姆加热对嗜酸耐热菌的杀灭作用机理。用自行设计的批式欧姆加热装置,对苹果汁中的嗜酸耐热菌进行处理后,利用扫描电镜观察其细胞壁膜结构的变化,利用电导率仪和紫外吸收分光光度计测定菌悬液的成分变化。结果表明:通过环境扫描电镜可观察到嗜酸耐热菌的表面出现凹陷和破损,电导率仪和紫外吸收分光光度计测定细胞内容物的溢出,可以推断出欧姆加热造成了嗜酸耐热菌的"电穿孔"。The microbe was treated by a self-made ohmic heating instrument to investigate the sterilization mechanism of ohmic heating on Alicyclobacillus acidoterrestris. The sterilization effect of ohmic heating on the microbe was observed by environmental scanning electron microscope （ESEM）. Conductometer and ultraviolet spectrophotometer was used to detect the leakage of intracellular contents. ESEM photographs of treated A. acidoterrestris showed that crushed and destroyed microbe resulted from the ohmic heating, the increase of electrical conductivity and the OD value indicate that the leakage of intracellular contents. It could be concluded that one of the sterilization mechanisms of ohmic heating on A. acidoterrestrls was electroporation.

关 键 词：欧姆加热 嗜酸耐热菌 电穿孔 

分 类 号：TS201.1[轻工技术与工程—食品科学]