离子色谱法测定食品中硝酸盐和亚硝酸盐  

Determination of Nitrate and Nitrite in Foods by Ion Chromatography

作　　者：张磊[1] 刘肖 赵云峰[1] 牟世芬 郑洪国 吴永宁[1] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所,北京100050 [2]戴安中国有限公司,北京100088

出　　处：《中国食品卫生杂志》2008年第4期294-297,共4页Chinese Journal of Food Hygiene

基　　金：“十一五”国家科技支撑计划食品安全关键技术重大项目,化学污染物暴露评估技术研究(2006BAK02A10)

摘　　要：目的建立同时测定食品中硝酸盐和亚硝酸盐的离子色谱方法。方法采用超声提取、固相萃取柱净化的方法对试样进行前处理,高容量阴离子交换色谱柱分离,抑制型电导检测器检测。结果亚硝酸盐和硝酸盐的检出限分别为0.005mg/L和0.008mg/L,回收率均在80%以上,RSD小于10%。结论该方法简便快捷、准确可靠,适用于多种食品基质中亚硝酸盐和硝酸盐的分析。Objective To establish a method for simultaneous determination of nitrite and nitrate in foods. Method Food samples were pre-treated by ultrasonic extraction, high-speed centrifugation and solid phase extract clean up, and the resulting supernatants were injected into a high-capacity anion exchange column with suppressed conductivity detection. Results The detection limits of nitrite and nitrate were 0.005 mg/L and 0.008 mg/L, respectively. Recovery rates were both above 80% and RSD for either ion was less than 10%. Conclusion The method could be simple, rapid, accurate and reliable, and suited for the analysis of nitrite and nitrate in various foods.

关 键 词：硝酸盐类 亚硝酸盐类 食品 色谱法 离子交换 

分 类 号：R155[医药卫生—营养与食品卫生学]