顶空固相微萃取气相色谱质谱法分析花牛苹果中的香气成分  

Determination of aroma components of the apple cultivar Malus pumila′Huaniu′by HS-SPME coupled with GC-MS

作　　者：魏玉梅[1] 周围[1] 毕阳[1] 

机构地区：[1]甘肃农业大学食品科学与工程学院,甘肃兰州730070

出　　处：《甘肃农业大学学报》2008年第4期135-139,共5页Journal of Gansu Agricultural University

摘　　要：用顶空固相微萃取（head-space solid-phase microextractions, HS-SPME）和气相色谱质谱（gas chromatgraphy mass spectrometrometry,GC-MS）联用技术对花牛苹果的香气成分进行了分析，共分离鉴定出了76种化学成分，其中酯类36种．占峰面积的45．71％；醇类13种，占峰面积的19．08％；醛类3种，占峰面积的9．62％；烯烃类3种，占峰面积的2．59％；其它物质占峰面积的12％，主要为酮类和烷烃类物质．选用OV-1701色谱柱、75μm CAR／PDMS萃取头，在萃取热平衡吸附时间25min，温度40℃，解吸附时间3min的试验条件下，得到的试验色谱峰保留时间和归一化面积的变异系数分别为0．08％～0．16％和2．72％～12．66％，且重复性良好．Aroma components in the fruits of the apple cultivar Malus pumila＇Huaniu＇were determined and analyzed by headspace solid-phase microextraction （HS-SPME） coupled with gas chromatography/ mass spectrometry（GC/MS）method. 76 kinds of chemical constituents were isolated and identified,including 36 kinds of lipid accounting for 45.71 % of peak area, 13 kinds of alcohols accounting for 19.08 % ,3 kinds of aldehydes accounting for 9.62 % ,3 kinds of alkenes accounting for 2.59 % ,and all the other volatile components accounting for 12 0%, such as ketones,alkanes and so on. The variation coefficients of retention time 12.66 under the of chlotoacetates and mormalized area were determined as 0. 08 % -0. 16 % and 2. 72% respectively, by using following experiment OV-1701 chromatographic column conditions, equilibrium adsorption and 75 μm CAR/PDMS extraction fiber time 25 min,temperature 40 ℃ and desorption time 3 min. The repeatability of the experiment was good.

关 键 词：顶空-固相微萃取 气相色谱-质谱联用 香气成分 花牛苹果 

分 类 号：Q658[生物学—生物物理学]