乳糖酶生产酵母菌菌株的研究  

Research the β-galactosidase to produce yeast fungus

作　　者：黄军[1] 熊华[1] 李亮[1] 李庭[1] 

机构地区：[1]南昌大学食品科学教育部重点实验室,江西南昌330047

出　　处：《江西食品工业》2008年第3期16-19,共4页Jiangxi Food Industry

摘　　要：从土壤和鲜牛奶中采用X-gal定性试验和ONPG定量试验对酶活性进行测定以筛选高产乳糖酶(β-半乳糖苷酶)的菌株。结果有21株在含有X-gal的琼脂培养基上产生蓝色菌落,5株ONPG酶活较高,其中酵母菌Y6号菌株酶活最高,酵母菌Y6号菌株在发酵培养20～24h生长量和产酶量均达到最大。By using X-gal test and ONPG test,the β-galactosidase activity quality and quantity were detected respectively,in order to screen the microorganism from soil and milk which produced large quantity of β-galactosidase. There were twenty-one strains that appeared blue colour on the agar plate containing X-gal. And there were five strains produced high quality and large quantity of β-galactosidase. And the β--galactosidase activity of yeast Y6 was the best of these five strains. And the Y6 strain of the yeast fungus had the biggest yield and productivity of lactase at 20-24h.

关 键 词：Β-半乳糖苷酶 菌种筛选 酵母菌 生长量产酶量 

分 类 号：TQ920.1[轻工技术与工程—发酵工程] Q949.326.1[生物学—植物学]