芭蕉芋淀粉和直链淀粉提取、分离、鉴定及含量测定  

Extraction,Purification,Characterization and Content Determination of Starch and Amylose from Canna edulis Ker. Growing in China

作　　者：张娟[1] 唐文凭[1] 王正武[1] 史贤明[1] 

机构地区：[1]上海交通大学农业与生物学院食品科学与工程系,上海200240

出　　处：《食品科学》2008年第9期303-306,共4页Food Science

基　　金：国家自然科学基金项目(20573048;20676051);上海市科委重大科技攻关项目(07DZ19508);上海市科委技术标准专项项目(07DZ05019)

摘　　要：本实验首先采用正交设计法优化芭蕉芋淀粉的提取工艺,并进行了实验验证,确定其最佳工艺为:料液比为1:2,静止时间为2h,洗涤次数为4次。然后采用热碱法和正丁醇-异戊醇结晶法制备得到了芭蕉芋直链淀粉的纯品,并对其从三个方面加以评定,得知碘-直链淀粉的最大紫外吸收波长为600nm、直链淀粉含量为87.19%、碘结合量为10.4。最后以制取得到的芭蕉芋直链淀粉和玉米支链淀粉为标准物制定标准曲线,测定得到了芭蕉芋淀粉中直链淀粉的含量为77.94%。这将对芭蕉芋淀粉的分子基础研究具有一定的意义。The optimized method for extraction and purification of Canna edulis Ker. starch was detenuined by orthogonal design and then validated by the experiment, and the maximum content of the starch was obtained when ratio of sample and liquid was 1：2, along with 2 hours of rest time and four times of washing. Then, the amylose of Canna edulis Ker. was extracted with the method of hot alkaline, and purified through n-butanol and isoamyl alcohol crystallization again and again. At last, absorption spectrum of iodin-amylose compound was scanned from 500 to 800 nm using UV spectrometer. The amylose content was determined by the method of hot alkaline and Fehling Reagent and iodine combined content of the amylose was determined by electrometric titration. The result showed that maximum absorption spectnun of the iodin-amylose compound locates at 600 um and the values of content and Iodine combined content of the amylose obtained are 87.14% and 10.4, respectively. In addition, the content of amylose in the Canna edulis Ker. starch is further determined as 77.94% through standard curve using the obtained amylose starch and amylopectin starch of corn as standards. This study can make a contribution to basic study of Canna edulis Ker. starch at molecular level.

关 键 词：芭蕉芋 淀粉 直链淀粉 工艺优化 含量测定 

分 类 号：TS235.9[轻工技术与工程—粮食、油脂及植物蛋白工程]