长裙竹荪乙醇提取工艺及抑菌作用研究  

Study on the extracted method of Dictyophora indusia fisscher and its antimicrobial action

作　　者：郝景雯[1] 张刚[1] 韩慧[1] 王东博[1] 何雨青[1] 

机构地区：[1]天津市工业微生物研究所,天津300462

出　　处：《食品工业科技》2008年第10期123-124,127,共3页Science and Technology of Food Industry

基　　金：天津市2006年度科委科技攻关项目(06YFGZNC00200)

摘　　要：以食用乙醇作为浸提溶剂,对长裙竹荪的有效抑菌成分进行浸提,确定了预处理工艺及酶解条件,即:竹荪干子实体粉细度为80目,超声波(45kHz)破碎处理20min,复合果胶酶制剂添加量为0.1%,酶解时间为1h。研究了浸提温度、浸提时间、料液比等因素对长裙竹荪有效成分提取率的影响,并通过正交实验确定了最佳提取工艺参数:浸提时间为8h,浸提温度为80℃,料液比为1:15。抑菌实验结果表明:长裙竹荪提取液对细菌有明显抑制作用,对酵母菌和霉菌抑制作用不明显。Using edible alcohol as a solvent, the extraction that had the antimicrobial characteristics from Dictyophora indusia fisscher was obtained. Through experiments,the optimal pre-extract process and the enzyme hydrolysis process were obtained： particle size of 80 head, ultrasonic （45kHz） broken with 20min, pectic enzyme adding 0.1%, enzyme hydrolysis time for th. By means of orthogonal design,the optimal extract process were obtained： extract time 8h, extract temperature 80℃, Dictyophora indusia fisscher particle： solvent menstruum = 1：15. The results showed that the extraction from Dictyophora indusia fisscher had strong antimicrobial effects on bacteria but weak on microzyme and mold.

关 键 词：长裙竹荪 食用乙醇 超声波处理 酶解工艺 抑菌作用 

分 类 号：TS201.2[轻工技术与工程—食品科学]