几种水解法制备高纯度大豆异黄酮甙元工艺比较研究  

A comparison of high-purity soybean isoflavone prepared by different hydrolysis methods

作　　者：黄爱妮[1] 汪海波[1] 

机构地区：[1]武汉工业学院食品科学与工程学院,湖北武汉430023

出　　处：《粮食与油脂》2008年第11期18-20,共3页Cereals & Oils

摘　　要：以低纯度商品大豆异黄酮为原料,利用酸水解法、超声波辅助酸水解法及酶水解法制备高纯度大豆异黄酮甙元产品。通过实验,比较这三种水解制备方法水解率、产品回收率和产品纯度,并对制备得到产品进行成分分析,选择出较佳制备高纯度大豆异黄酮甙元方法。实验结果表明,采用β―葡萄糖苷酶水解分离纯化工艺制备大豆异黄酮甙元产品,操作简单,安全环保,其产品得率、异黄酮回收率和产品纯度分别为15.1%、77.1%和92.7%,分离纯化效果较理想。The high purification soy isoflavone aglycone was extracted from low purification soy isoflavone product by acid hydrolysis, ultrasound-assisted acid hydrolysis and enzyme hydrolysis. In this study, in order to choose a better method to prepare high-purity soybean isoflavone, the hydrolysis rates, the isoflavone returns-ratio and the product purity of the three hydrolysis methods are compared, and the composition of the product is analyzed. The results showed that it＇ s easy and safe to separate and purify soybean isoflavone by β-glucosidase hydrolysis method, and the product obtains rate is 15.1%, the isoflavone returns-ratio is 77.16%, the product purity is 92.78%.

关 键 词：大豆异黄酮 酸水解 超声波 酶水解 

分 类 号：TS202.3[轻工技术与工程—食品科学]