啤酒花超临界CO2萃余物中总黄酮的提取与抗氧化活性研究  

Study on Extraction Techology and Antioxidant Activity of Total Flavonoids from Supercritical CO_2 Extration Residue of Humulus lupulus

作　　者：杨小兰[1] 郑海鹰[1] 胡仕屏[1] 罗正明[1] 张晓云[2] 

机构地区：[1]山西大学生命科学与技术学院,山西太原030006 [2]山西医科大学第一医院呼吸科,山西太原030001

出　　处：《食品科学》2008年第11期137-141,共5页Food Science

基　　金：山西省自然科学基金项目(2006011019);太原市大学生创新创业计划项目(08122014)

摘　　要：通过单因素和正交试验确定了啤酒花CO2萃余物中总黄酮的最佳提取条件:浸提剂50%乙醇,料液比1:25(g/ml),回流提取温度70℃,提取时间2.5h,在此条件下总黄酮的提取量为68.09mg/g干酒花。抗氧化实验结果显示提取的啤酒花总黄酮对DPPH自由基的清除能力(IC505.73μg/ml)优于抗坏血酸(IC508.79μg/ml)和芦丁(IC5014.84μg/ml);啤酒花总黄酮的还原力(EC500.158mg/ml),优于芦丁(EC500.252mg/ml),弱于抗坏血酸(EC500.103mg/ml);啤酒花总黄酮对小鼠肝匀浆Fe2++H2O2诱导脂质过氧化产生丙二醛(MDA)的抑制能力(IC500.517mg/ml)优于芦丁(IC500.596mg/ml),表明啤酒花总黄酮具有显著的抗氧化活性。The extraction technology of total flavonoids from supercritical CO2 extraction residue ofHumulus lupulus was optimized by using singel factor and orthogonal test. The optimal extraction conditions are determined as follows： 50% ethanol as extraction solvent, the ratio of materiel to 50% ethanol 1： 25（g/ml）, circumfluence temperature 70℃, and circumfluence time 2.5 h, and extraction rate reaches 68.09 mg/g dry Humulus lupulus under the above conditions. The results ofantioxidative experiment showed that the flavonoids extracts has stronger DPPH freed radical scavenging activity （IC50 5.73 μg/ml） than ascorbic acid （IC50 8.79 μg/ml ） and rutin（14.84 μg/ml）, and it has stronger reducing capacity （EC50 0.158 mg/ml） than rutin （0.252 mg/ml）, but weaker than ascorbic acid （0.103 mg/ml）; It also has stronger inhibition rate on MDA produced by lipideroxidation induced by Fe^2＋-H2O2 in mouse liver homogenate than rutin. As a conclusion, the total flavonoids from supercritical CO2 extraction residue ofHumulus lupulus has strong antioxdant capacity.

关 键 词：超临界CO2萃余物 啤酒花总黄酮 提取工艺 DPPH自由基 还原力 脂质过氧化 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]