窖泥和黄泥微生物分离分析的培养基选择及评价  

Selection of and Evaluation on the Isolation Medium for Microbes in Pit Mud and Clay Soil

作　　者：齐慧[1] 邓依[1] 张磊[1] 李红[1] 张文学[1] 胡峰 李波 杨开梅 

机构地区：[1]四川大学轻纺与食品学院,四川成都610065 [2]贵州茅台集团习酒责任有限公司,贵州习水564622

出　　处：《酿酒科技》2009年第1期58-61,共4页Liquor-Making Science & Technology

摘　　要：对黔型浓香型白酒习酒的发酵窖泥及环境土样中微生物区系构成情况进行调查分析,选择多种分别针对细菌、放线菌、酵母菌、丝状真菌等微生物菌群的分离培养基进行了调查探讨。实验结果显示:添加1600u/mL青霉素钠的培养基可完全抑制原核类微生物菌落的长出,添加0.067%孟加拉红的培养基可抑制部分真菌的扩散生长;选择葡萄糖胰胨琼脂培养基、高氏一号培养基、YEPD培养基或卡尔氏培养基、孟加拉红培养基或淀粉培养基或查氏培养基等,分别用于分离培养细菌、放线菌、酵母菌、丝状真菌等微生物菌群,可获得较好的分离效果和获得更多种群的菌落。在方法学上,采用适合的分离培养基探讨窖泥和黄泥中微生物区系的基本构成是可行的。In order to investigate the microflora in fermented pit mud of Luzhou-flavor liquor and in enviromental clay soil, the isolation medium for bacteria, actinomyces, yeasts and filamentous fungi were selectively used. The experimental results showed that the addition of 1600 u/mL sodium penicillin in isolation medium could completely inhibit the growth of prokaryotic microbes, the addition of 0.067 % rose-bengal in isolation medium could inhibit the growth and the spread of part of filamentous fungi, and the selection of desoxycholate medium, Gause＇s synthetic medium, YEPD medium or Carr＇s medium, Rose-bengal medium or starch medium or Czapek＇s medium could successfully isolate bacterium, actinomyces, yeasts and filamentous fungi respectively, and could achieve better isolation effects and obtain more microflora. Accordingly, it was feasible that the use of proper isolation medium to study the basic constitution of microflora in pit mud and in clay soil.

关 键 词：浓香型白酒 窖泥 黄泥 培养基选择 微生物种群 

分 类 号：TS262.31[轻工技术与工程—发酵工程] TS261.4[轻工技术与工程—食品科学与工程]