全酶法制备甘油二酯的研究(Ⅱ)——脂肪酸酶法酯化反应的工艺优化  

Production of diacylglycerol using holoenzymatic process(Ⅱ):optimization of enzymatic esterification of fatty acids

作　　者：杨继国[1] 杨博[2] 王永华[1] 宁正祥[1] 

机构地区：[1]华南理工大学轻工与食品学院,广州510641 [2]华南理工大学生物科学与工程学院,广州510641

出　　处：《中国油脂》2009年第3期15-19,共5页China Oils and Fats

基　　金：国家自然科学基金青年基金项目(20506007)

摘　　要：通过对酶催化部分水解反应和酶催化酯化反应的有效整合,提出了全酶法制备甘油二酯的工艺,主要就工艺中的脂肪酸酶法酯化反应进行了研究。以油脂部分水解反应所得到的脂肪酸为底物,探讨了反应形式、底物配比、反应体系温度、进料流速等因素对反应的影响,并对酯化反应产物进行分子蒸馏提纯。较优的反应条件为:脂肪酶Lipozyme RMIM,采用填充床酶反应器连续酯化的反应形式,脂肪酸与甘油的摩尔比为1∶1,进料流速1.5 mL/min,反应温度60℃,此时脂肪酸转化率达到76.2%,产物经过分子蒸馏提纯后,可以获得含量达到92.2%的甘油二酯产品。填充床酶反应器连续运行10 d后,Lipozyme RMIM的催化活力仍能保持在80%以上。The holoenzymatic process combining the enzymatic controlled hydrolysis with enzymatic esterification was developed to produce diacylglycerol. Optimization of enzymatic esterification of fatty acids, part of the holoenzymatic process,was studied. Fatty acids obtained from the partial hydrolysis of oil was used as a reaction substrate, and the influences of factors on reaction, including the mode of reaction, molar ratio of fatty acid to glycerol, reaction temperature, flow rate and so on, were decided, and the product of esterification reaction was further purified by molecular distillation. 76.2% conversion of fatty acids was observed under the following optimal conditions ： lipase Lipozyme RM IM, packed - bed enzymatic reactor used in esterification, molar ratio of fatty acid to glycerol 1：1, reaction temperature 60 ℃, flow rate 1.5 mL/min. After molecular distillation, the product with 92.2% DAG was obtained. Above 80% enzymatic activity of Lipozyme. RM IM was reserved after 10 days continuous operation in packed -bed enzymatic reactor.

关 键 词：甘油二酯 脂肪酶 酯化 填充床酶反应器 分子蒸馏 

分 类 号：TQ645.9[化学工程—精细化工]