几种抗冻剂对养殖大黄鱼蛋白质冷冻变性抑制作用的研究  

Inhibition of frozen denaturation of cultured Pseudosciaena Crocea protein by several antifreezes

作　　者：崔雁娜 戴志远 张志广 王宏海 

机构地区：[1]浙江工商大学水产品加工研究所,浙江杭州310035

出　　处：《食品工业科技》2009年第3期68-70,187,共4页Science and Technology of Food Industry

基　　金：浙江省海洋开发管理项目(04-09);浙江省重大科技攻关项目(2005C12014);国家863项目(2007AA091803)。

摘　　要：以养殖大黄鱼为原料,通过添加多聚磷酸盐、山梨醇、海藻糖,进行正交实验,研究pH、盐溶性蛋白含量和Ca2+-ATPase活性的变化,得出防止蛋白质冷冻变性的三种抗冻剂的最佳配比。实验结果表明:三种抗冻剂的最佳配比为:2%多聚磷酸盐、1%海藻糖、2%山梨醇,在添加抗冻剂后,养殖大黄鱼的pH、盐溶性蛋白质含量、ATPase活力分别比未添加抗冻剂时提高了1.60%、33.98%、43.52%。To study the optimum effectiveness in preventing the frozen denaturation of cultured Pseudosciaena Crocea protein by three kinds of antifreezes, polyphosphate,trehalose, sorbitol were added, orthogonal design was used,pH,salt dissolved protein content and Ca^2＋-ATPase activity were used as indexes.The results showed：the optimum design was polyphosphate 2% ,trehalose 1%,sorbitol 2%.When antifreezes were added, pH,salt dissolved protein content, Ca^2＋- ATPase activity of cultured Pseudosciaena Crocea were improved by 1.60%, 33.98% and 43.52%.

关 键 词：养殖大黄鱼 蛋白质冷冻变性 抗冻剂 正交实验 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]