猪皮胶原粉的制备工艺及功能特性研究  

Preparation Techniques of Pigskin Collagen Powder and Its Functional Characteristics

作　　者：韩德权[1] 金明姬[1] 吴桐[1] 曹相辉[1] 

机构地区：[1]黑龙江大学,黑龙江哈尔滨150080

出　　处：《食品科学》2009年第10期60-64,共5页Food Science

基　　金：黑龙江省科技厅2006年攻关项目(GB06B403-2)

摘　　要：本研究在4℃条件下用弱酸加酶法提取可溶性胶原并干燥成粉,然后对已提取过的猪皮用60%乙醇溶液作载体进行胶体磨研磨,制备不溶性胶原粉。进一步对其进行胶原含量及功能特性的测定。结果表明:猪皮胶原粉的最佳制备工艺为:采用pH2.0醋酸+0.2%胃蛋白酶、料液比1:15条件下提取猪皮48h,可溶性胶原溶出率为0.602%;剩余猪皮采用60%乙醇载体研磨后干燥可制得不溶性胶原粉。所得胶原粉各项功能特性优良,通过羟脯氨酸含量测定得出可溶性胶原粉胶原纯度为95.1%,不溶性胶原粉胶原纯度为82.9%,工艺路线易于实现规模化生产。Pigskin soluble collagen was prepared by enzymolysis in addition of weak acid at 4 ℃, and then dried into powder, and the remaining pigskin was further grinded in the presence of ethanol to prepare insoluble collagen powder. The extraction technology of soluble collagen was optimized by orthogonal test, and the ethanol concentration for preparing insoluble collagen powder was selected. Besides, the collagen content and functional properties of the two collagen powders were analyzed. Results showed that the addition of acetic acid was able to enhance the swelling of pigskin and the dissolution rate of soluble collagen. The optimal extraction conditions of soluble collagen with pepsin were as follows： enzyme concentration 0.2%, solid to liquid ratio 1：15, pH 2.0 and hydrolysis time 48 h, and under the above conditions the yield of soluble collagen was 0.602%. 60% ethanol was the best for preparing insoluble collagen powder. The collagen contents of obtained soluble collagen powder and insoluble collagen powder were 95.1% and 82.9%, respectively. Both the two collagen powders exhibited good functional characteristics. The results suggested the feasibility of this technology for the production in plant scale.

关 键 词：猪皮胶原 胃蛋白酶 功能特性 

分 类 号：TS209[轻工技术与工程—食品科学]