酶解法生产降血压肽的水产蛋白原料筛选研究  

Screening of Aquatic Protein Material for ACEIIP Producing by Enzyme Hydrolysis

作　　者：王金玲[1] 李恒星[1] 董晓美[1] 陈启和[1] 冯凤琴[1] 何国庆[1] 

机构地区：[1]浙江大学生物系统工程与食品科学学院,杭州310029

出　　处：《中国食品学报》2009年第3期111-116,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：浙江省重大招标项目(No.2006C12096)

摘　　要：目的:筛选适于酶解法生产降血压肽的水产蛋白原料。方法:在分析15种水产品与水产品下脚料基本成分的基础上,以酶解法(酸性蛋白酶和碱性蛋白酶)酶解15种原料,测定不同酶解液的水解度、ACE体外抑制率和ACEIC50。结果:碱性蛋白酶对原料的水解度较大;15种原料的酶解液均表现出不同的ACE抑制效果,抑制率在19%～99%之间;以ACEIC50为评价指标,ACE抑制效果好的原料有鲫鱼、鲈鱼、带鱼脊骨和鲳鱼,ACE-IC50分别为11.68、12.96、11.26、11.18mg/mL(酸性蛋白酶酶解)。结论:综合考虑降血压肽的工业化生产要素和ACE抑制活性,选取价格低廉、原料预处理简单的带鱼脊骨作为ACE抑制肽生产的水产蛋白原料。Objective： The objective of this study was to screen suitable material for ACEIP producing. Method： Based on the analysis and comparison of components in 15 kinds of different aquatic products and its by-products, DH, ACE inhibitory rate and ACEIC50 of hydrolysates by two different enzymes： acidic protease and alcalase were assayed. Results： The results showed that DH of alcalase was higher than that of acidic protease. The hydrolysates of 15 materials by two enzymes inhibited ACE in different levels; inhibitory rates were from 19% to 99%. Among 15 kinds of materials, crucian carp, Japanese sea bass, ribbonfish backbone, and silvery pomfret showed better ACEIC50, the values were 11.68,12.96,11.26,11.18 mg/mL by acidic protease hydrolysis, respectively. Conclusion ： As concerning of industrialization, ribbonfish backbone is the most suitable material for its cheap and easy pretreatment characteristics.

关 键 词：酶解 水产蛋白 ACE抑制肽 筛选 

分 类 号：TQ464.7[化学工程—制药化工]