毛壳霉CQ31的鉴定及固体发酵产木聚糖酶条件的优化  

Identification of Chaetomium sp. CQ31 and Optimization of Xylanase Production in Solid State Fermentation

作　　者：丛倩千[1] 江正强[1] 吕顺意[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《微生物学通报》2009年第8期1269-1274,共6页Microbiology China

基　　金：国家自然科学基金资助项目(No.30500099);高等学校全国优秀博士学位论文作者专项资金(No.200555)

摘　　要：从土壤中筛选出一株产木聚糖酶的真菌CQ31,经鉴定后命名为毛壳霉CQ31。该菌能够利用几种农业废弃物固体发酵高产木聚糖酶,玉米杆为最佳碳源。单因素优化试验表明:以玉米杆为碳源,胰蛋白胨为氮源,初始水分含量80%,初始pH值9.0为最佳产酶条件。在优化后的条件下培养7d产木聚糖酶水平高达4897U/g干基碳源,此时甘露聚糖酶酶活达803U/g干基碳源。因此,毛壳霉CQ31固体发酵产木聚糖酶和甘露聚糖酶具有一定的工业化应用前景。A fungus CQ31 isolated from soil samples was identified as Chaetomium sp.. This strain produced effectively xylanases utilizing several liguocellulosic materials in the solid-state fermentation （SSF）, and corn straw was the best carbon source. The results of single-factor-experiment showed that the corn straw as the carbon source, tryptone as the nitrogen source, initial moisture content of 80% and initial pH 9.0 were the optimal conditions for xylanase production. Under the optimized conditions, it produced xylanase which was 4897 U/g dry substrate while mannanase was 803 U/g dry substrate after 7 days of cultivation. Therefore, xylanase and mannanase production by Chaetomium sp. CQ31 in SSF possess potential for commercial applications.

关 键 词：毛壳霉 木聚糖酶 甘露聚糖酶 固体发酵 玉米杆 

分 类 号：TQ925[轻工技术与工程—发酵工程]