响应面法优化L.lactis WH11-1生成GABA的发酵条件  

Process Optimization of Fermentation Conditions of GABA by Response Surface Analysis

作　　者：罗斌[1] 孟和毕力格[1] 冀林立[1] 张彦斌[1] 杜小华[1] 宿淑辉[1] 赵俊[1] 

机构地区：[1]内蒙古农业大学食品科学与工程学院乳品生物技术与工程教育部重点实验室,内蒙古呼和浩特010018

出　　处：《微生物学杂志》2009年第4期53-58,共6页Journal of Microbiology

基　　金：内蒙古农业大学博士基金项目(K31610)

摘　　要：通过响应面分析的方法对L.lactis WH11-1发酵生产γ-氨基丁酸的培养条件进行了优化。运用中心组合设计和响应面分析考察了接种量、初始pH值和培养温度对γ-氨基丁酸产量的影响,得出最佳培养条件:接种量4.5%,初始pH值为6.0,培养温度为32℃。在此条件下培养96 h,GABA生成量达8.63 g/L,实验值与预测值基本相符。Response surface analysis （RSA） was applied to optimize the cultivation conditions for y-Aminobutyric Acid（GABA） production. Different factors which had effect on production of y-amino butyric acid, such as the inoculating amount, initiational pH, culture temperature were studied by a central composite design and response surface analysis. The results showed that the content of GABA was 8.63 g/L in the optimum cultivation conditions, which were inoculation volume 4.5% , the initiation pH at 6.0, for 96 h at 32℃, the experimental values agreed with the predicted values.

关 键 词：Γ-氨基丁酸 发酵条件 优化 响应面分析法 

分 类 号：Q939[生物学—微生物学]