毕赤酵母基因工程菌高密度发酵产纳豆激酶条件研究  

High cell density fermentation conditions of nattokinase by genetic engineering strain Pichia pastoris GS115/pPRONK1

作　　者：闫达中[1] 许芳[1] 杨艳燕[2] 

机构地区：[1]武汉工业学院生物与制药工程学院,湖北武汉430023 [2]湖北大学生命科学学院,湖北武汉430062

出　　处：《中国酿造》2009年第9期59-61,共3页China Brewing

摘　　要：高密度发酵是提高发酵产量的一个非常有效的手段。通过摇瓶培养确定毕赤酵母基因工程菌(GS115/pPRONK1)生长和表达纳豆激酶的最优化条件,并在此基础上利用5 L发酵罐进行分批补料高密度培养,确定最适发酵条件,即接种量10%,发酵最适pH值为8.0,表达的最佳温度为25℃,甲醇诱导18 h后,纳豆激酶的最高活力达到6.5×104U/L。High cell - density fermentation is a very effective approach to improve production yield. The optimal fermentation conditions of genetic engineering strain Pichia pastoris GS115/pPRONK1 were investigated in flask, and the fed - batch high density cuhrure was carried out on 5L fermentor. The optimal fermentation conditions were as follows： the inoculum 10%,pH 8.0, temperature 25℃. Under these conditions, the nattokinase activity reached 6.5 ×10^4 U/L after induced by methanol for 18 h.

关 键 词：纳豆激酶 基因工程菌 高密度发酵 

分 类 号：TQ925[轻工技术与工程—发酵工程]