检测黄曲霉毒素生物合成相关基因芯片的研制  

Development of Gene Chip for Aflatoxin Detection

作　　者：胡娜[1] 许杨[2] 涂追[2] 

机构地区：[1]江西科技师范学院生命科学学院,江西南昌330013 [2]南昌大学中德联合研究院,江西南昌330047

出　　处：《食品科学》2009年第19期185-189,共5页Food Science

基　　金：江西科技师范学院青年创新基金项目

摘　　要：研究比较成熟的可用于分析黄曲霉毒素生物合成相关基因的芯片分析技术。芯片点阵后依次通过温浴2h,650mJ/cm2紫外交联30s、80℃烘烤2h、预杂交45min、清洗、干燥等步骤,最后与待测样品在42℃杂交16h,可获得低背景高质量的芯片。本实验所用探针来源于寄生曲霉,而待测样品选择了黄曲霉菌株,芯片扫描结果显示荧光信号稳定,与反转录PCR扩增结果一致,并且无背景干扰。证明探针设计合理,实验方法可靠。初步建立了用芯片检测与黄曲霉毒素生物合成相关基因的技术平台。In order to detect genes related to aflatoxin biosynthesis, a high quality gene chip was successfully developed here. The preparation of this gene chip was involved in a series of processes： array on the slide, heating in 42 ℃ water bath for 2 h, UV-induced crosslink at 650 mJ/cm^2 for 30 seconds, baking in oven at 80℃ for 2 h, pre-hybridization for 45 min, then washing and drying, and final hybridization with fluor-derivatized samples at 42℃ for 16 h. The probe originated from Aspergillus parasiticus could be used to analyze aflatoxin gene expression in Aspergillusflavus. Therefore, this probe can provide stable fluorescence signal without background inference and results consistent with reverse transcriptase polymerase chain reaction （RT-PCR）.

关 键 词：寄生曲霉 黄曲霉 黄曲霉毒素 基因芯片 

分 类 号：Q789[生物学—分子生物学]