没食子酸微晶纤维素酯的生物抗氧化研究  

Bio-antioxidation Mechanism of Gallic Acid Microcrystalline Cellulose Ester

作　　者：刘宁[1,2] 方桂珍[1] 陈姝娟[2] 苏霜[2] 李健[2] 

机构地区：[1]东北林业大学生物质材料科学与技术教育部重点实验室,黑龙江哈尔滨150040 [2]哈尔滨商业大学食品工程学院,黑龙江哈尔滨150076

出　　处：《食品科学》2009年第17期312-315,共4页Food Science

基　　金：高等学校博士学科点专项科研基金资助项目(200602258);黑龙江省省教育厅科学技术研究面上项目(11541078)

摘　　要：以微晶纤维素、没食子酸为原料,采用间接酯化法合成没食子酸微晶纤维素酯。采用体外动物实验对没食子酰微晶纤维素酯抗氧化性能进行评价,并进行急性毒理学研究。结果显示:不同浓度的没食子酰微晶纤维素酯均可明显地提高小鼠肝组织GSH-Px活力,同时还可显著抑制H2O2诱导的脂质过氧化产物丙二醛(MDA)的生成和红细胞的氧化溶血,且呈极显著的量效关系。没食子酸微晶纤维素酯雌雄小鼠LD50大于10.00g/kgbw,属无毒级物质,与原料没食子酸、已作为食品抗氧化剂使用的没食子酸丙酯相比,安全性有所提高。In order to solve safety problems in the use of gallic acid as a food antioxidant, gallic acid microcrystalline cellulose ester, a new-type high-molecular-weight antioxidant which has high antioxidant activity, was synthesized by indirect esterification method from gallic acid and microcrystalline cellulose which is safe and can not be easily absorbed by the human body. The in vitro andoxidant activities of gallic acid microcrystalline cellulose ester were evaluated and its acute toxicity in mice was studied. Gallic acid microcrystalline cellulose ester at different concentrations could significantly improve the GSH-Px activity of mouse liver tissue and inhibit the generation of lipid peroxidation product （MDA） induced by I-hO2 and the oxidative hemolysis of erythrocyte in a dose-effect manner. The acute oral LD50 for male and female mice was more than 10.00 g/kg bw, proving that gallic acid microcrystalline cellulose ester is a non-toxic compound which has higher safety than gallic acid and propyl gaUate which has been used as a food antioxidant.

关 键 词：没食子酸 微晶纤维素 没食子酸微晶纤维素酯 抗氧化 

分 类 号：TQ943[化学工程] TQ352