姜油树脂的超临界CO_2萃取及其抗氧化性研究  

Supercritical CO_2 Fluid Extraction and Antioxidant Activity of Ginger Oleoresin

作　　者：郑君成[1] 高兆建[1] 刘辉[1] 蒋永红 何海洋[1] 王冉[1] 唐仕荣[1] 

机构地区：[1]徐州工程学院食品工程学院,江苏徐州221008 [2]徐州技源天然保健品有限公司,江苏徐州221008

出　　处：《食品科学》2009年第18期163-167,共5页Food Science

基　　金：徐州工程学院2007年度自然科学研究计划项目(XKY2007223);江苏省2008年大学生实践创新计划项目(苏教高[2008]34号)

摘　　要：以鲜生姜为原料,通过单因素和正交试验对姜油树脂超临界萃取工艺中萃取压力、萃取温度、萃取时间、物料颗粒的大小和夹带剂五个影响萃取效果的因素进行探讨,确定姜油树脂的最佳萃取工艺:鲜生姜50℃远红外干燥,粉碎过120目筛,35MPa,35℃条件下萃取2.5h,质量提取率6.08%,姜辣素提取率2.78%。抗氧化活性实验表明,超临界CO2萃取的姜油树脂具有一定的清除DPPH自由基的能力,清除能力与浓度呈较明显的量效关系。气相色谱-质谱联用(GC-MS)分析鉴定出76种化合物,其中16种属于姜辣素,相对含量达33.95%,表明超临界CO2萃取的姜油树脂中富含姜辣素等抗氧化活性成分。Ginger oleoresin was extracted from dried ginger powder using supercritical CO2 fluid with the addition of absolute ethanol as a colsolvent. The effects of extraction pressure, extraction temperature, extraction time, particle size of material and amount of absolute ethanol on extraction yields of ginger oleoresin and gingerols were investigated by single factor and orthogonal array methods. The optimum conditions for ginger oleoresin extraction were determined as the follows： particle size of dried material,below 120 mesh; extraction pressure, 35 MPa; extraction temperature, 35 ℃, and extraction time 2.5 h. Under these conditions, the extraction yield of ginger oleoresin and gingerols reached 6.08% and 2.78%, respectively. Ginger oleoresin could effectively scavenge DPPH free radicals in a significant dose-dependent manner. GC-MS analysis indicated that there were 76 compounds identified in ginger oleoresin, of which 16 ones might belong to the gingerol family and the relative content was 33.95%. These results suggest that ginger oleoresin is rich in antioxidant components as gingerols.

关 键 词：姜油树脂 超临界CO2 工艺研究 抗氧化性 气相色谱-质谱法 

分 类 号：TS201.2[轻工技术与工程—食品科学]