粗糙脉孢菌木聚糖酶酶学性质的研究  

Properties of xylanase from Neurospora crassa

作　　者：赵亮亮[1,2] 李燕荣[3] 梁鲜菊[1,2] 谌斌[1,2] 

机构地区：[1]广西环境工程与保护评价重点实验室,广西桂林541004 [2]广西师范大学环境与资源学院,广西桂林541004 [3]中国农业科学院农业资源与农业区划研究所,北京100081

出　　处：《中国酿造》2009年第10期26-28,共3页China Brewing

基　　金：广西环境工程与保护评价重点实验室研究基金(桂科能0702Z022)

摘　　要：对粗糙脉孢菌木聚糖酶粗酶的酶学性质进行了研究,结果表明:该木聚糖酶的最适反应温度为50℃、pH值为5.8,温度低于50℃、pH4.6～7.4时该酶稳定性较好;金属离子Ca2+、Mg2+、Mn2+、Fe2+对该酶的活性有促进作用,K+、Na+、Pb2+、Cu2+、Fe3+、Al3+对酶的活性有抑制作用;EDTA、SDS、DMSO对该酶活性的抑制作用较小;以木聚糖为底物的酶动力学常数Km和Vmax值分别为1.30g/L和1.35μmol(/min·mL)。The properties of xylanase from Neurospora crassa were studied in this paper. The results showed that the optimal reaction temperature and pH value of the enzyme were 50℃ and 5.8, respectively. The activity of xylanase was relatively stable below 50℃ and at the pH range of 4.6～7.4. Its activity was activated by Ca^2＋, Mg^2＋, Mn^2＋, and Fe^2＋, but inhibited by K^＋, Na^＋, Pb^2＋, Cu^2＋, Fe^3＋, Al^3＋, EDTA, SDS, and DMSO. The kinetics coefficients of Km and Vmax with xylan as substrate were 1.30 g/L and 1.35 μmol/（min· mL）, respectively.

关 键 词：粗糙脉孢菌 木聚糖酶 酶学性质 

分 类 号：Q556[生物学—生物化学]