响应曲面法优化从桑叶中酶法提取芦丁新工艺  

Optimization of enzymatic extraction process of rutin from mulberry (Morus alba L.) leaves using response surface methodology

作　　者：张金亮[1] 杨清[2] 王明伟[1] 王蒙[1] 张霄[1] 王俊[1] 吴福安[1,3] 张建平[1] 

机构地区：[1]江苏科技大学生物与环境工程学院,江苏镇江212018 [2]西南大学食品科学学院,重庆400715 [3]中国农业科学院蚕业研究所,江苏镇江212018

出　　处：《食品工业科技》2009年第11期211-214,共4页Science and Technology of Food Industry

基　　金：江苏省高校自然科学基础研究面上项目(08KJB530002);江苏省高等学校大学生实践创新训练计划2008年立项项目(340);现代农业产业技术体系建设专项资金资助;江苏科技大学本科生创新计划专项经费资助

摘　　要：以桑叶为原料,建立酶法提取芦丁的新工艺。以芦丁提取率为评价指标,单因素法获取酶解工艺参数的优化区间,响应曲面法优化酶法提取芦丁的工艺参数。实验结果表明,从桑叶中酶法提取芦丁的最佳工艺条件为:酶解温度30℃,纤维素酶浓度0.5mg/mL,酶解介质pH3.0,处理时间7h。酶法从桑叶中提取芦丁的平均提取率为0.786mg/g,相比传统乙醇提取法提高了60.5%。Using mulberry （Morus alba L.） leaves as raw material,adopting an enzymatic extraction method to study a new production process of rutin.Extraction yield of rutin was taken as the evaluation index,optimization range of the enzymatic extraction process parameters were selected using single factor methodology,then those best conditions were optimized using response surface methodology.Experiment results showed that when the enzymatic hydrolysis temperature was 30℃,the concentration of enzyme was 0.5mg /mL, the pH value was 3.0, and the enzymatic treatment time was 7h, the optimum extraction rate of rutin from mulberry leaves was 0.786mg/g, which was 60.5% higher than that in traditional alcohol extraction method.

关 键 词：桑叶 芦丁 酶法提取 工艺优化 响应曲面法 

分 类 号：TS201.2[轻工技术与工程—食品科学]