不同碳、氮源对猴头菌菌丝体多糖含量的影响  

Effects of Different Carbon and Nitrogen Sources on Content of Polysaccharide in Liquid Submerged-fermented Mycelia and Broth of Hericium erinaceus

作　　者：汪敬健[1] 温鲁[1] 翁梁[1] 吉鸿辉 孙娟[1] 

机构地区：[1]淮阴师范学院资源微生物研究所,江苏淮安223300

出　　处：《食品科学》2010年第1期149-151,共3页Food Science

基　　金：江苏省大学生实践创新计划立项资助项目;淮阴师范学院教授基金项目(06HSJS027)

摘　　要：目的:明确培养猴头菌菌丝体时不同碳、氮源对多糖含量的影响。方法:用不同碳、氮源液体培养基对猴头菌进行液体浅层静置培养,用苯酚-硫酸法测定菌丝体中的多糖含量。结果:从菌丝体多糖含量角度考虑最佳碳源为淀粉,最佳氮源为豆腐渣;在淀粉用量为4.0%、豆腐渣用量为1.5%时得到的猴头菌菌丝体多糖含量高达162.17mg/g。结论:淀粉和豆腐渣能比常用碳、氮源大幅度提高液体培养猴头菌菌丝体的多糖含量,而且价格低、来源广,在生产上有着很好的推广应用前景。In order to exploit new carbon and nitrogen sources for Hericium erinaceus,effects of kind and amount of carbon and nitrogen sources on the content of polysaccharide in harvested mycelia and broth of Hericium erinaceus after 35-day liquid submerged fermentation.The phenol-sulfuric acid method was used to determine polysaccharide content.Results showed that the optimal carbon and nitrogen sources were starch and soybean dregs for polysaccharide production in Hericium erinaceus mycelia,respectively,and the content of polysaccharide was as high as 162.17 mg/g when the amounts of starch and soybean dregs were 4.0% and 1.5%.In conclusion,starch and soybean dregs are the optimal choices of carbon and nitrogen sources for polysaccharide production in Hericium erinaceus due to low cost and broad source,which will have a promising popularilization prospect in practical production.

关 键 词：猴头菌 多糖 液体静置培养 苯酚-硫酸法 

分 类 号：TS201.3[轻工技术与工程—食品科学] S646[轻工技术与工程—食品科学与工程]