苹果渣固态发酵产复合酶培养基优化的研究  

Optimization of Solid-state Fermentation Medium for Production of Complex Enzymes with Apple Pomace

作　　者：姜心[1] 陈伟[1] 周波[2] 

机构地区：[1]山东农业大学食品科学与工程学院,山东泰安271018 [2]山东农业大学生命科学学院,山东泰安271018

出　　处：《山东农业科学》2010年第1期80-85,共6页Shandong Agricultural Sciences

基　　金：国家科技基础条件平台"微生物菌种资源整理整合项目(2005DKA21201-13)";国家科技支撑计划"黄淮平原区农田秸秆资源综合利用技术集成研究与示范(2007BAD89B09-16)"资助

摘　　要：以黑曲霉C-2为菌种,对苹果渣进行固态发酵,以纤维素酶、木聚糖酶、果胶酶的活性为测定指标,单因素试验确定水平,采用L9(34)正交试验对产复合酶体系的固态培养基质进行优化,同时运用极差分析、方差分析以及不同因素水平间差异显著性检验对结果进行分析,以得到较合理的培养基。优化后培养基组成为果渣∶麸皮=4∶1,2.0%硫酸铵,0.1%磷酸氢二钾,2.5%尿素。优化后三种酶活力比基础培养基分别提高了64.19%、17.26%、20.89%。With the activity of cellulase, xylanase and pectinase as the determination indicators, the optimization of medium composition was investigated in solid - state fermentation of apple pomace by Aspergillus niger C -2. The culture medium was optimized using L9 （3＆4） orthogonal design, range analysis, variance analysis and test of significance between the levels of different factors , so as to get a more reasonable result. As the result, the optimized medium composition was apple pomace： bran = 4： 1, ammonium sulfate of 2.0%, potassium hydrogen phosphate of 0. 1%, urea of 2.5%. Compared with the basic medium, the activity of three enzyme was increased by 64.19%, 17.26% and 20.89%, respectively.

关 键 词：苹果渣 固态发酵 复合酶 培养基 优化 

分 类 号：TS202.3[轻工技术与工程—食品科学]