枯草芽孢杆菌TF2磺胺胍抗性基因的克隆和表达  

CLONING AND EXPRESSION OF SULFAGUANIDINE RESISTANT GENE OF BACILLUS SUBTILIS TF2

作　　者：王艳萍[1] 张克旭[1] 陈宁[1] 刘淑云[1] 李干[1] 

机构地区：[1]天津轻工业学院

出　　处：《南开大学学报（自然科学版）》1998年第4期35-39,共5页Acta Scientiarum Naturalium Universitatis Nankaiensis

摘　　要：采用鸟枪法将枯草芽孢杆菌（Ｂａｃｉｌｕｓｓｕｂｔｉｌｉｓ）磺胺胍抗性基因（ＳＧｒ）克隆到质粒ｐＵＢ１１０中建成重组质粒ｐＢＵＷ４，该质粒的分子量约８．１ｋｂ，标记为ＳＧｒ和Ｋｍｒ．将ｐＵＢＷ４转化到肌苷产生菌枯草芽孢杆菌Ｑ２９０１－４菌株中，得到的转化子产肌苷能力比受体菌提高２５．５％．Sulfaguanidine resistant (SG r) gene of Bacillus subtilis was cloned in plasmid pUB110 using shotgun method. The molecular weight of the recombinant plasmid pUBW4 containing SG r gene was about 8.1kb and its genetic markers were SG r and Km r. pUBW4 was transformed into inosine producing B.subtilis strain Q2901 4. The yield of inosine of the transformant B. subtilis strain Q2901 4 35 (pUBW4) was 25.5% higher than that of the receptor strain.

关 键 词：枯草芽孢杆菌 磺胺胍抗性基因 克隆 肌苷 发酵 

分 类 号：TQ929[轻工技术与工程—发酵工程]