酵母转化茄尼醇生成辅酶Q_(10)的超临界CO_2体系条件  

Supercritical CO_2 Conditions of CoQ_(10) Biotransformation from Solanesol by Schizosaccharomyces prombe

作　　者：肖霄[1] 倪元颖[1] 李淑燕[1] 胡锦荣[1] 张京声[1] 孙君社[1] 刘萍[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《食品与生物技术学报》2010年第2期302-306,共5页Journal of Food Science and Biotechnology

基　　金：北京市自然科学基金项目(5062022)

摘　　要：超临界CO2作为非水相介质对酵母转化茄尼醇生成辅酶Q10的底物和产物具有良好的溶解作用,但对微生物的存在会产生一定影响,选择合适的超临界CO2体系条件有利于辅酶Q10产量的提高。通过研究超临界CO2流体的压强、酵母细胞在超临界CO2流体中的反应时间、添加介质种类以及添加量等条件对粟酒裂殖酵母细胞活性和辅酶Q10产量的影响,确定对酵母细胞生存影响较小并显著促进辅酶Q10生产的超临界CO2体系条件为:超临界CO2压强为10.5 MPa,反应时间为16 h,向菌体中添加菌体湿重3倍的培养基,超临界CO2流体中辅酶Q10的产量达到55.71μg/mL。比液体深层发酵培养辅酶Q10产量提高97.5%。研究结果表明,通过优化酵母转化茄尼醇生成辅酶Q10的超临界CO2体系条件,可以显著提高酵母细胞生成辅酶Q10的能力。Supercritical CO2（scCO2） as non-aqueous media could well dissolve substrates and products of coenzyme Q10（CoQ10） biotransformation reaction from solanesol,but it also had effects on microbial activity,so appropriate scCO2 conditions could improve CoQ10 yield.The effects of scCO2 on the catalytic activity of Schizosaccharomyces prombe（S.promb） and CoQ10 yield in different conditions were studied.The appropriate scCO2 conditions which could significantly promote the production of CoQ10 and could have little negative effects on the cells survival was： triple of cells＇ wet weight of culture medium was added into the reaction system.,kept the pressure at 10.5 MPa for 16 h,the production of CoQ10 is 55.71 μg/mL,improved 97.5%.The results showed that optimization of scCO2 reaction system conditions could significantly improve the production of CoQ10.

关 键 词：超临界CO2流体 菌体活性 辅酶Q10 茄尼醇 体系条件 

分 类 号：TQ464.8[化学工程—制药化工]