枯草芽孢杆菌BS-05产纤维素酶条件的研究  

Study on the cellulase producing conditions of the Bacillus subtilis BS-05

作　　者：涂宗财[1] 江国忠[1] 刘益东[1] 刘成梅[1] 张秋婷[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047

出　　处：《食品工业科技》2010年第5期181-183,187,共4页Science and Technology of Food Industry

基　　金：教育部长江学者和创新团队发展计划项目(IRT0540)

摘　　要：以实验室筛选的枯草芽孢杆菌产纤维素酶菌株BS-05为出发菌株,对其产纤维素酶条件进行了研究。实验结果表明,菌株BS-05产纤维素酶的最佳条件是:以2%的CMC-Na为碳源,2%的蛋白胨和2%的酵母粉为氮源,产酶培养基初始pH为7,装液量为60mL/250mL,接种量5%,在37℃,150r/min下培养48h。在此条件下,该菌株产酶能力最强,羧甲基纤维素酶活力CMCA达195.46U/mL,滤纸酶活力FPA达174.52U/mL。Laboratory screening cellulase producing bacterial strain BS-05 was the purpose bacterial strain,the best conditions for its production of cellulase was studied. The experiment results were：2% CMC-Na as the carbon source,2% peptone and 2% dusty yeast as the nitrogen source,the initial fermentation medium pH was adjusted to 7,liquid volume was 60mL/250mL,inoculum size was 5%,37℃,cultivated 48h at 150r/min. Under these conditions,its enzyme producing ability was the strongest,in the crude extract,cellulose enzyme activity was the highest,carboxy methyl cellulase CMCA activity reached to 195.46U/mL,filter paper enzyme activity FPA reached to 174.52U/mL.

关 键 词：枯草芽孢杆菌 纤维素酶 产酶条件 优化 

分 类 号：TS201.25[轻工技术与工程—食品科学]