Folin-Ciocalteu法测定甘蔗糖厂制品的总酚含量  

Determination of total phenolics in cane sugar product by Folin-Ciocalteu colorimetry

作　　者：林金梅[1] 卢家炯[1,2] 赵迎春[1] 

机构地区：[1]广西大学轻工与食品学院,南宁530004 [2]广西大学糖业工程技术研究中心,南宁530004

出　　处：《食品科技》2010年第6期278-280,共3页Food Science and Technology

基　　金：广西研究生教育创新计划项目

摘　　要：用Folin-Ciocalteu比色法,以没食子酸为标准品,分析测定了甘蔗糖厂生产制品的总酚含量。试验数据表明,总酚含量在0～6μg/mL范围内具有良好线性关系,标准曲线的回归方程为:Y=0.0959X(R2=0.9992);对稳定性、精密度、回收率进行了试验,加标回收率98.10%～100.20%,RSD为0.85%,为进一步分析检测甘蔗糖制品提供了依据。The total phenolics content of cane sugar products were analyzed by Folin-Ciocalteu colorimetry.The experiment was investigated using gallic acid as standard.The study showed that the content of total phenolics had a good linearity between the absorbance and gallic acid concentration from 0～6 μg/mL in 765 nm,and regressive equation of standard curve is Y=0.0959X（R2=0.9992）.The recoveries and the relative standard deviation values were in the range of 98.10%～100.20% and 0.32%～1.88% respectively.This method was simple,accurate and appropriate to measure the total phenolics content of cane sugar products.

关 键 词：糖制品 酚类物 F-C比色法 

分 类 号：TS247[轻工技术与工程—制糖工程]