水酶法提取青稞蛋白工艺研究  

Study on extraction of hulless barley protein using aqueous enzymatic method

作　　者：王金水[1] 李涛[1] 焦健[1] 

机构地区：[1]河南工业大学粮油食品学院,河南郑州450052

出　　处：《食品工业科技》2010年第9期267-269,共3页Science and Technology of Food Industry

摘　　要：以纤维素酶作为水解酶,采用水酶法从青稞米籽粒中提取蛋白质。通过单因素实验和正交实验,得到提取青稞蛋白的最佳条件,即料/水比1:5、加酶量0·2%、酶解时间14h、酶解温度45℃、酶解pH6·5。在最佳条件下青稞蛋白的提取率为69·3%。The aqueous enzymatic method was used to extract hulless barley protein.The food-grade cellulase was adopted.Based on the single-factor and orthogonal experiments,the optimal extraction conditions were obtained as follows：sample/water ratio of 1：5,0.2% of cellulase（enzyme/sample）,hydrolyzing for 14h at 45℃ and pH 6.5.The extraction rate of hulless barley protein could amount to 69.3% under the optimal conditions.

关 键 词：青稞 蛋白质 纤维素酶 水酶法 

分 类 号：TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程]