固定化细胞技术进行乳酸发酵生产的研究  

Study on Lactic Acid Fermentation With Immobilized Cells

作　　者：陶静[1] 尧俊英[1] 李洁[1] 

机构地区：[1]郑州轻工业学院食品与生物工程学院,河南郑州450002

出　　处：《科学之友（下）》2010年第9期146-147,共2页Friend of Science Amateurs

摘　　要：文章以乳酸菌为菌种,活化培养后固定于海藻酸钠、琼脂、明胶-戊二醛三种载体上,分别选取不同的接种量、胶珠直径、载体浓度、CaCl2浓度等因素设计单因素实验和正交实验,以36h为时间范围恒温下进行发酵;通过还原糖量和酸度的测定,衡量不同条件对乳酸产量的影响,最终选出最佳发酵工艺条件。In this paper,the strains of lactic acid bacteria was fixed in three carriers respectively after be activated,that is sodium alginate,agar,gelatin-glutaraldehyde. By selecting different inoculums size,diameter of plastic beads,carrier concentration,CaCl2 concentration,I done single factor experiments and orthogonal test in 36 h time frame for the fermentation to carried out under constant temperature; By mensurate the reducing sugar and LA degrees,we can determine the effects of fermentation in different conditions,and ultimately select the best conditions.

关 键 词：乳酸发酵 固定化细胞 凝胶包埋法 

分 类 号：TQ921[轻工技术与工程—发酵工程]