Alcalase碱性蛋白酶水解厚壳贻贝蛋白  

Study on Hydrolysis of Mytilus coruscus Protein with Alcalase Protease

作　　者：戴志远[1] 张婷[1] 张燕平[1] 卢延斌[1] 张艳萍[1] 

机构地区：[1]浙江工商大学水产品加工研究所,学士教授浙江杭州310012

出　　处：《食品与发酵工业》2010年第8期83-88,共6页Food and Fermentation Industries

基　　金：浙江省厅市会商项目(2008C02013)

摘　　要：以厚壳贻贝为原料,Alcalase2.4L碱性蛋白酶为酶源,在单因素试验的基础上,以还原力A700为指标,运用响应面分析法(RSM)得到Alcalase2.4L酶解厚壳贻贝蛋白的优化条件为:底物浓度[S]3.2%、[E]/[S]2.12%、温度55.54℃、pH8.66、时间2h,该条件下制备的酶解物的还原能力为0.587。同时证明了该酶解物具有显著清除DPPH自由基活性和较强的超氧阴离子自由基清除能力。Alcalase2.4L were applied to hydrolyze the Mytilus coruscus protein. Based on the results of single- factor tests, reducing power AT00 was chosen as the response values. Using Response Surface Methodology （RSM） , the optimum enzyme hydrolysis conditions of Alcalase2.4L to achieve the maximum antioxidant ability were as follows ： substrate concentration （ [ S] ） 3.20%, [ E ]/[ S]2. 12%, enzymolysis temperature 55.54℃, and enzymolysis time 2 hours. Under such conditions, reducibility of the hydrolysate reached 0. 587, which also had strong scavenging activity of DPPH radical and superoxide anion radical.

关 键 词：厚壳贻贝 酶解 抗氧化 响应面分析法 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]